Detailed information of TA system TA108343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 5316..5935
• Replicon: plasmid pDEF-2
• Accession: NZ_CP031029
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: DTO64_RS16495
• Protein ID: WP_000241511.1
• Coordinates: 5316..5678 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: DTO64_RS16500
• Protein ID: WP_000245205.1
• Coordinates: 5672..5935 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS16480	726..2657	-	1932	WP_002370258.1	sucrose-specific PTS transporter subunit IIBC	-
DTO64_RS16485	2848..4287	+	1440	WP_002367771.1	sucrose-6-phosphate hydrolase	-
DTO64_RS16490	4289..5251	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
DTO64_RS16495	5316..5678	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DTO64_RS16500	5672..5935	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
DTO64_RS16505	6112..6732	+	621	WP_021732893.1	recombinase family protein	-
DTO64_RS16510	6749..7036	+	288	WP_010708491.1	hypothetical protein	-
DTO64_RS16515	7030..7254	+	225	WP_010708492.1	hypothetical protein	-
DTO64_RS16520	7315..7524	+	210	WP_002387638.1	hypothetical protein	-
DTO64_RS16525	7536..7838	+	303	WP_002365939.1	hypothetical protein	-
DTO64_RS16530	8265..8678	+	414	Protein_11	excinuclease ABC subunit A	-
DTO64_RS16535	8741..9346	-	606	WP_002321606.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	asa1	1..58791	58791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T108343 WP_000241511.1 NZ_CP031029:c5678-5316 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T108343 NZ_CP031029:c5678-5316 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT108343 WP_000245205.1 NZ_CP031029:c5935-5672 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT108343 NZ_CP031029:c5935-5672 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1