108342

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 47548..48082 Replicon plasmid pDEF-1
Accession NZ_CP031028
Organism Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag DTO64_RS16300 Protein ID WP_002360769.1
Coordinates 47807..48082 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag DTO64_RS16295 Protein ID WP_002369771.1
Coordinates 47548..47814 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO64_RS16260 43651..44436 + 786 WP_000803546.1 replication-relaxation family protein -
DTO64_RS16265 44442..44930 + 489 WP_002368088.1 hypothetical protein -
DTO64_RS16275 45522..45788 + 267 WP_002368091.1 hypothetical protein -
DTO64_RS16280 45821..46315 + 495 WP_002368092.1 DnaJ domain-containing protein -
DTO64_RS16285 46413..46829 + 417 WP_002368093.1 single-stranded DNA-binding protein -
DTO64_RS16290 46853..47437 + 585 WP_002368095.1 thermonuclease family protein -
DTO64_RS16295 47548..47814 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DTO64_RS16300 47807..48082 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
DTO64_RS16310 48885..49070 + 186 WP_002368099.1 hypothetical protein -
DTO64_RS16315 49092..49238 + 147 WP_002368101.1 DpnD/PcfM family protein -
DTO64_RS16320 49469..50248 + 780 WP_002368102.1 hypothetical protein -
DTO64_RS16325 50248..51507 + 1260 WP_002368103.1 PcfJ domain-containing protein -
DTO64_RS16335 52157..52885 + 729 WP_002368105.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III - 1..75758 75758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T108342 WP_002360769.1 NZ_CP031028:47807-48082 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T108342 NZ_CP031028:47807-48082 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT108342 WP_002369771.1 NZ_CP031028:47548-47814 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT108342 NZ_CP031028:47548-47814 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References