Detailed information of TA system TA108341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 9190..9859
• Replicon: plasmid pDEF-1
• Accession: NZ_CP031028
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: DTO64_RS16040
• Protein ID: WP_010708525.1
• Coordinates: 9464..9859 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: DTO64_RS16035
• Protein ID: WP_002331065.1
• Coordinates: 9190..9462 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS15995	4489..5397	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
DTO64_RS16000	5394..5936	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
DTO64_RS16005	6029..6823	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
DTO64_RS16015	7099..7671	+	573	WP_001079938.1	HTH domain-containing protein	-
DTO64_RS16025	7969..8865	+	897	WP_002334978.1	ParA family protein	-
DTO64_RS16030	8964..9173	+	210	WP_114679443.1	peptide-binding protein	-
DTO64_RS16035	9190..9462	+	273	WP_002331065.1	antitoxin	Antitoxin
DTO64_RS16040	9464..9859	+	396	WP_010708525.1	zeta toxin family protein	Toxin
DTO64_RS16045	9887..10567	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
DTO64_RS16050	10601..10852	-	252	WP_010708530.1	hypothetical protein	-
DTO64_RS16055	10895..11563	-	669	WP_002383592.1	CPBP family intramembrane metalloprotease	-
DTO64_RS16060	11694..12707	-	1014	WP_002387529.1	replication initiator protein A	-
DTO64_RS16065	12967..13323	-	357	WP_002360663.1	hypothetical protein	-
DTO64_RS16070	13316..14098	-	783	WP_002369746.1	ParA family protein	-
DTO64_RS17005	14352..14642	-	291	WP_114679444.1	type III secretion system protein PrgN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III	-	1..75758	75758
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / aph(3')-III	-	493..23315	22822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T108341 WP_010708525.1 NZ_CP031028:9464-9859 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

>T108341 NZ_CP031028:9464-9859 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT108341 WP_002331065.1 NZ_CP031028:9190-9462 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT108341 NZ_CP031028:9190-9462 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55