Detailed information of TA system TA108311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2586849..2587535
• Replicon: chromosome
• Accession: NZ_CP031027
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: DTO64_RS13590
• Protein ID: WP_114679421.1
• Coordinates: 2587191..2587535 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: DTO64_RS13585
• Protein ID: WP_174089957.1
• Coordinates: 2586849..2587172 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS13530	2582368..2583183	-	816	WP_162781818.1	PD-(D/E)XK nuclease-like domain-containing protein	-
DTO64_RS13535	2583146..2584156	-	1011	WP_114679414.1	recombinase RecT	-
DTO64_RS13545	2584257..2584481	-	225	WP_002367281.1	hypothetical protein	-
DTO64_RS13550	2584478..2584777	-	300	WP_114679415.1	hypothetical protein	-
DTO64_RS13555	2584844..2585023	-	180	WP_114679416.1	hypothetical protein	-
DTO64_RS13560	2585058..2585327	-	270	WP_002367284.1	hypothetical protein	-
DTO64_RS13565	2585403..2585717	+	315	WP_114679417.1	hypothetical protein	-
DTO64_RS13575	2585842..2586018	-	177	WP_002385636.1	helix-turn-helix domain-containing protein	-
DTO64_RS13580	2586106..2586384	+	279	WP_114679419.1	hypothetical protein	-
DTO64_RS17000	2586381..2586542	-	162	WP_174089958.1	hypothetical protein	-
DTO64_RS13585	2586849..2587172	+	324	WP_174089957.1	helix-turn-helix domain-containing protein	Antitoxin
DTO64_RS13590	2587191..2587535	+	345	WP_114679421.1	ImmA/IrrE family metallo-endopeptidase	Toxin
DTO64_RS13595	2587594..2588322	+	729	WP_114679422.1	potassium channel family protein	-
DTO64_RS13600	2588477..2589658	+	1182	WP_114679423.1	site-specific integrase	-
DTO64_RS13605	2589761..2589910	-	150	WP_002356321.1	50S ribosomal protein L33	-
DTO64_RS13610	2590036..2592291	-	2256	WP_174222123.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2554723..2589658	34935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13608.64 Da
• Isoelectric Point: 5.6177

>T108311 WP_114679421.1 NZ_CP031027:2587191-2587535 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNIIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T108311 NZ_CP031027:2587191-2587535 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAATTAAAGC
ATGTAAAAGACCATGTGGATATAATGGCATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAAGCTGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATATTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12292.87 Da
• Isoelectric Point: 5.2520

>AT108311 WP_174089957.1 NZ_CP031027:2586849-2587172 [Enterococcus faecalis]
MTVFDRVKKLADSQKISIVELEEKLNFSRNSLYAWKKSKPSIDKLEAVANYFGVSTDYLLGREVSNKSKQSDDLDEVLDN
VMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 324 bp

>AT108311 NZ_CP031027:2586849-2587172 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTAAAAAAATTAGCAGATAGTCAGAAAATATCTATTGTCGAACTTGAAGAAAAGTTAAATTT
CAGTCGAAATTCATTATACGCTTGGAAAAAAAGTAAACCTTCTATTGATAAATTAGAAGCTGTTGCAAATTATTTTGGAG
TTTCAACGGATTATTTATTAGGTCGTGAAGTTTCTAATAAATCAAAGCAATCTGATGATTTGGATGAAGTATTAGACAAT
GTGATGAGTTTCGATGGTGAACCTTTGGATGATCATGATCGGGAAGTTATTCGTGCATATTTAAAGGGTAGATTCGGGAA
ATAA