Detailed information of TA system TA108310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 416849..417345
• Replicon: chromosome
• Accession: NZ_CP031027
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3GZA4
• Locus tag: DTO64_RS02240
• Protein ID: WP_002358470.1
• Coordinates: 417076..417345 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R3JPM7
• Locus tag: DTO64_RS02235
• Protein ID: WP_002413218.1
• Coordinates: 416849..417076 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS02210	412993..413523	+	531	WP_002358466.1	hypothetical protein	-
DTO64_RS02215	413538..414362	+	825	WP_002358467.1	hypothetical protein	-
DTO64_RS02220	414738..415001	+	264	WP_002358468.1	hypothetical protein	-
DTO64_RS02225	414994..415551	+	558	WP_002358242.1	hypothetical protein	-
DTO64_RS02230	415554..416789	+	1236	WP_002358469.1	hypothetical protein	-
DTO64_RS02235	416849..417076	+	228	WP_002413218.1	antitoxin	Antitoxin
DTO64_RS02240	417076..417345	+	270	WP_002358470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DTO64_RS02245	417412..418992	+	1581	WP_002404807.1	DUF3991 domain-containing protein	-
DTO64_RS02250	419009..419713	+	705	WP_010706734.1	hypothetical protein	-
DTO64_RS02255	419739..420158	+	420	WP_025186145.1	TnpV protein	-
DTO64_RS02260	420296..420559	+	264	WP_123839658.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 10.5395

>T108310 WP_002358470.1 NZ_CP031027:417076-417345 [Enterococcus faecalis]
MSYRLEFTPDVKKQLKKMDKYQATLLTRWLYRNIDGTANPREHGKALSANRAGQWRYRIGNYRVIVEIEDERLVVTAIQV
GHRRNVYDK

Nucleotide

• Length: 270 bp

>T108310 NZ_CP031027:417076-417345 [Enterococcus faecalis]
ATGTCCTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8785.90 Da
• Isoelectric Point: 4.0179

>AT108310 WP_002413218.1 NZ_CP031027:416849-417076 [Enterococcus faecalis]
MAMITVRVSDEEKEWLSYMAEFFGVTLSDLLKSYSMEQLEDEYDKQTAEIAHKRWIEQGKETISMDEILNEFGGL

Nucleotide

• Length: 228 bp

>AT108310 NZ_CP031027:416849-417076 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Structures

Toxin

• AlphaFold DB: R3GZA4

Antitoxin

• AlphaFold DB: R3JPM7