Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 144333..144975 Replicon chromosome
Accession NZ_CP030982
Organism Bacillus cereus strain ZB201708

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag DT426_RS00850 Protein ID WP_000635965.1
Coordinates 144625..144975 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DT426_RS00845 Protein ID WP_000004570.1
Coordinates 144333..144620 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DT426_RS00820 139648..140610 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
DT426_RS00825 140603..141175 - 573 WP_000908529.1 rhomboid family intramembrane serine protease -
DT426_RS00830 141269..141628 + 360 WP_000583418.1 holo-ACP synthase -
DT426_RS00835 141785..142735 + 951 WP_003276258.1 outer membrane lipoprotein carrier protein LolA -
DT426_RS00840 142853..144022 + 1170 WP_000390595.1 alanine racemase -
DT426_RS00845 144333..144620 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DT426_RS00850 144625..144975 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DT426_RS00855 145043..147211 + 2169 WP_000426209.1 RNA-binding transcriptional accessory protein -
DT426_RS00860 147269..147385 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
DT426_RS00865 147581..148039 + 459 WP_000343553.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T108290 WP_000635965.1 NZ_CP030982:144625-144975 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T108290 NZ_CP030982:144625-144975 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT108290 WP_000004570.1 NZ_CP030982:144333-144620 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT108290 NZ_CP030982:144333-144620 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References