Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3732254..3732872 Replicon chromosome
Accession NZ_CP030939
Organism Escherichia coli strain AMSHJX01

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DTO54_RS19740 Protein ID WP_001291435.1
Coordinates 3732654..3732872 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DTO54_RS19735 Protein ID WP_000344800.1
Coordinates 3732254..3732628 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO54_RS19725 3727343..3728536 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DTO54_RS19730 3728559..3731708 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
DTO54_RS19735 3732254..3732628 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DTO54_RS19740 3732654..3732872 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DTO54_RS19745 3733043..3733594 + 552 WP_000102564.1 maltose O-acetyltransferase -
DTO54_RS19750 3733710..3734180 + 471 WP_000136192.1 YlaC family protein -
DTO54_RS19755 3734344..3735894 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DTO54_RS19760 3735936..3736289 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DTO54_RS19770 3736668..3736979 + 312 WP_000409911.1 MGMT family protein -
DTO54_RS19775 3737010..3737582 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108261 WP_001291435.1 NZ_CP030939:3732654-3732872 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T108261 NZ_CP030939:3732654-3732872 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT108261 WP_000344800.1 NZ_CP030939:3732254-3732628 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT108261 NZ_CP030939:3732254-3732628 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References