108250

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1366382..1367007 Replicon chromosome
Accession NZ_CP030939
Organism Escherichia coli strain AMSHJX01

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag DTO54_RS07080 Protein ID WP_000911329.1
Coordinates 1366609..1367007 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag DTO54_RS07075 Protein ID WP_000450524.1
Coordinates 1366382..1366609 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO54_RS07050 1362184..1362654 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
DTO54_RS07055 1362654..1363226 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
DTO54_RS07060 1363372..1364250 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
DTO54_RS07065 1364267..1365301 + 1035 WP_001440783.1 outer membrane protein assembly factor BamC -
DTO54_RS07070 1365514..1366227 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
DTO54_RS07075 1366382..1366609 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
DTO54_RS07080 1366609..1367007 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
DTO54_RS07085 1367154..1368017 + 864 WP_001267498.1 neutral zinc metallopeptidase -
DTO54_RS07090 1368032..1370047 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
DTO54_RS07095 1370121..1370819 + 699 WP_000679823.1 esterase -
DTO54_RS07100 1370929..1371129 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T108250 WP_000911329.1 NZ_CP030939:1366609-1367007 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T108250 NZ_CP030939:1366609-1367007 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT108250 WP_000450524.1 NZ_CP030939:1366382-1366609 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT108250 NZ_CP030939:1366382-1366609 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References