Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 914098..914752 Replicon chromosome
Accession NZ_CP030939
Organism Escherichia coli strain AMSHJX01

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag DTO54_RS04825 Protein ID WP_000244772.1
Coordinates 914345..914752 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DTO54_RS04820 Protein ID WP_000354046.1
Coordinates 914098..914364 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO54_RS04795 909267..910010 + 744 WP_000951964.1 SDR family oxidoreductase -
DTO54_RS04800 910067..911500 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
DTO54_RS04805 911545..911856 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
DTO54_RS04810 912020..912679 + 660 WP_000250274.1 hemolysin III family protein -
DTO54_RS04815 912875..913855 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DTO54_RS04820 914098..914364 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DTO54_RS04825 914345..914752 + 408 WP_000244772.1 protein YgfX Toxin
DTO54_RS04830 914792..915313 - 522 WP_001055874.1 flavodoxin FldB -
DTO54_RS04835 915425..916321 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DTO54_RS04840 916346..917056 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DTO54_RS04845 917062..918795 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T108248 WP_000244772.1 NZ_CP030939:914345-914752 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T108248 NZ_CP030939:914345-914752 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT108248 WP_000354046.1 NZ_CP030939:914098-914364 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT108248 NZ_CP030939:914098-914364 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References