Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 262060..262691 Replicon chromosome
Accession NZ_CP030939
Organism Escherichia coli strain AMSHJX01

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag DTO54_RS01285 Protein ID WP_001259385.1
Coordinates 262060..262335 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag DTO54_RS01290 Protein ID WP_000593554.1
Coordinates 262332..262691 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO54_RS01270 257064..258131 + 1068 WP_000361467.1 HlyD family secretion protein -
DTO54_RS01275 258128..260863 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
DTO54_RS01280 260863..261987 + 1125 WP_001314210.1 ABC transporter permease -
DTO54_RS01285 262060..262335 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
DTO54_RS01290 262332..262691 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DTO54_RS01295 262741..263601 - 861 WP_001131783.1 class II aldolase -
DTO54_RS01300 263633..263902 - 270 WP_000933060.1 HPr family phosphocarrier protein -
DTO54_RS01305 263892..265400 - 1509 WP_001298718.1 carbohydrate kinase -
DTO54_RS01310 265393..266751 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
DTO54_RS01315 266828..267109 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
DTO54_RS01320 267106..267579 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T108246 WP_001259385.1 NZ_CP030939:262060-262335 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T108246 NZ_CP030939:262060-262335 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT108246 WP_000593554.1 NZ_CP030939:262332-262691 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT108246 NZ_CP030939:262332-262691 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References