Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509964..510600 Replicon chromosome
Accession NZ_CP030937
Organism Bacillus sp. DM2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DS740_RS02600 Protein ID WP_003156187.1
Coordinates 510250..510600 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag DS740_RS02595 Protein ID WP_003225183.1
Coordinates 509964..510245 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS740_RS02575 506324..506923 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
DS740_RS02580 507018..507383 + 366 WP_015252768.1 holo-ACP synthase -
DS740_RS02585 507549..508565 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
DS740_RS02590 508679..509848 + 1170 WP_015252766.1 alanine racemase -
DS740_RS02595 509964..510245 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DS740_RS02600 510250..510600 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DS740_RS02605 510715..511539 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
DS740_RS02610 511544..511909 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
DS740_RS02615 511913..512314 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
DS740_RS02620 512326..513333 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
DS740_RS02625 513395..513724 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
DS740_RS02630 513721..514203 + 483 WP_003234299.1 anti-sigma B factor RsbW -
DS740_RS02635 514169..514957 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
DS740_RS02640 514957..515556 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T108226 WP_003156187.1 NZ_CP030937:510250-510600 [Bacillus sp. DM2]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T108226 NZ_CP030937:510250-510600 [Bacillus sp. DM2]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT108226 WP_003225183.1 NZ_CP030937:509964-510245 [Bacillus sp. DM2]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT108226 NZ_CP030937:509964-510245 [Bacillus sp. DM2]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References