Detailed information of TA system TA108225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 56684..57821
• Replicon: plasmid pCR1B
• Accession: NZ_CP030934
• Organism: Enterococcus gilvus strain CR1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EGCR1_RS18655
• Protein ID: WP_114526085.1
• Coordinates: 56958..57821 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: EGCR1_RS18650
• Protein ID: WP_000301765.1
• Coordinates: 56684..56956 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGCR1_RS18625	52162..52533	+	372	WP_012477193.1	DUF3021 family protein	-
EGCR1_RS18630	52597..53214	+	618	WP_114526082.1	recombinase family protein	-
EGCR1_RS18635	53214..55367	+	2154	WP_114526083.1	type IA DNA topoisomerase	-
EGCR1_RS18640	55506..56360	+	855	WP_114526084.1	ParA family protein	-
EGCR1_RS18645	56458..56667	+	210	WP_000527317.1	peptide-binding protein	-
EGCR1_RS18650	56684..56956	+	273	WP_000301765.1	antitoxin	Antitoxin
EGCR1_RS18655	56958..57821	+	864	WP_114526085.1	zeta toxin family protein	Toxin
EGCR1_RS18660	58262..58579	+	318	WP_002338433.1	hypothetical protein	-
EGCR1_RS18665	58599..59096	+	498	WP_114526086.1	molecular chaperone DnaJ	-
EGCR1_RS18670	59130..59435	+	306	WP_114526087.1	hypothetical protein	-
EGCR1_RS18675	59458..59715	-	258	WP_002325623.1	hypothetical protein	-
EGCR1_RS18680	59718..60017	-	300	WP_002325624.1	hypothetical protein	-
EGCR1_RS18685	60309..62294	+	1986	WP_114526088.1	MobA/MobL family protein	-
EGCR1_RS18690	62318..62650	+	333	WP_000713503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80244	80244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32462.02 Da
• Isoelectric Point: 6.6618

>T108225 WP_114526085.1 NZ_CP030934:56958-57821 [Enterococcus gilvus]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDKGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T108225 NZ_CP030934:56958-57821 [Enterococcus gilvus]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATAAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTACAAG
CCAAAGGTTATGAAACAAAAATGTACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT108225 WP_000301765.1 NZ_CP030934:56684-56956 [Enterococcus gilvus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT108225 NZ_CP030934:56684-56956 [Enterococcus gilvus]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3