Detailed information of TA system TA108221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 175724..176460
• Replicon: plasmid pKC-Pl-HB1
• Accession: NZ_CP030924
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KC-Pl-HB1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: DTN91_RS27460
• Protein ID: WP_004098919.1
• Coordinates: 175978..176460 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: DTN91_RS27455
• Protein ID: WP_004213599.1
• Coordinates: 175724..175990 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTN91_RS27430	170957..171514	+	558	WP_004213590.1	recombinase family protein	-
DTN91_RS27435	171517..174486	+	2970	WP_004213592.1	Tn3 family transposase	-
DTN91_RS27440	174528..175022	+	495	WP_004213594.1	hypothetical protein	-
DTN91_RS27445	175083..175286	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
DTN91_RS27450	175300..175530	+	231	WP_004213598.1	hypothetical protein	-
DTN91_RS27455	175724..175990	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
DTN91_RS27460	175978..176460	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
DTN91_RS27465	176881..178108	+	1228	Protein_190	IS3 family transposase	-
DTN91_RS27470	178104..178499	-	396	Protein_191	DDE-type integrase/transposase/recombinase	-
DTN91_RS27480	178674..179696	-	1023	WP_004214536.1	porphobilinogen synthase	-
DTN91_RS27485	179760..181106	-	1347	WP_011154555.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..196000	196000
	-	inside	IScluster/Tn	-	-	170957..178108	7151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T108221 WP_004098919.1 NZ_CP030924:175978-176460 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T108221 NZ_CP030924:175978-176460 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT108221 WP_004213599.1 NZ_CP030924:175724-175990 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT108221 NZ_CP030924:175724-175990 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A7D3T1D0