Detailed information of TA system TA108220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 110339..111009
• Replicon: plasmid pKC-Pl-HB1
• Accession: NZ_CP030924
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KC-Pl-HB1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DTN91_RS27090
• Protein ID: WP_004213072.1
• Coordinates: 110566..111009 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DTN91_RS27085
• Protein ID: WP_004213073.1
• Coordinates: 110339..110569 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTN91_RS27060	106562..107461	-	900	WP_004225022.1	nucleotide-binding protein	-
DTN91_RS27065	107451..107741	-	291	WP_004213078.1	hypothetical protein	-
DTN91_RS27070	108093..108299	+	207	WP_004213077.1	hypothetical protein	-
DTN91_RS27075	108289..108582	-	294	WP_004213076.1	hypothetical protein	-
DTN91_RS27080	108598..109731	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DTN91_RS27085	110339..110569	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DTN91_RS27090	110566..111009	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DTN91_RS27095	111023..111439	-	417	Protein_123	restriction endonuclease	-
DTN91_RS27100	111443..111736	-	294	Protein_124	transposase	-
DTN91_RS28705	112153..112788	+	636	Protein_125	response regulator	-
DTN91_RS27120	113303..113709	-	407	Protein_126	GAF domain-containing protein	-
DTN91_RS27130	113800..114720	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
DTN91_RS27135	114769..115260	-	492	WP_011251327.1	DM13 domain-containing protein	-
DTN91_RS27140	115323..115598	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..196000	196000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T108220 WP_004213072.1 NZ_CP030924:110566-111009 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T108220 NZ_CP030924:110566-111009 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT108220 WP_004213073.1 NZ_CP030924:110339-110569 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT108220 NZ_CP030924:110339-110569 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620