Detailed information of TA system TA108219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 90377..91128
• Replicon: plasmid pKC-Pl-HB1
• Accession: NZ_CP030924
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KC-Pl-HB1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: DTN91_RS26970
• Protein ID: WP_004902249.1
• Coordinates: 90646..91128 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: DTN91_RS26965
• Protein ID: WP_004902250.1
• Coordinates: 90377..90655 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTN91_RS26930	85405..85695	+	291	WP_011154627.1	MSMEG_0570 family nitrogen starvation response protein	-
DTN91_RS26935	85713..86978	+	1266	WP_004210292.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
DTN91_RS26940	86959..88629	+	1671	WP_004902261.1	AMP-binding protein	-
DTN91_RS28700	88867..89136	+	270	WP_071591895.1	hypothetical protein	-
DTN91_RS26955	89597..89938	+	342	WP_004902257.1	hypothetical protein	-
DTN91_RS26960	90046..90258	+	213	WP_004902255.1	hypothetical protein	-
DTN91_RS26965	90377..90655	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
DTN91_RS26970	90646..91128	+	483	WP_004902249.1	GNAT family N-acetyltransferase	Toxin
DTN91_RS26985	92163..92702	+	540	WP_004902239.1	hypothetical protein	-
DTN91_RS26990	92807..93199	+	393	WP_045145491.1	hypothetical protein	-
DTN91_RS26995	93300..94055	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
DTN91_RS27000	94082..94729	+	648	WP_014386537.1	EcsC family protein	-
DTN91_RS27005	94861..95097	-	237	Protein_105	kinase	-
DTN91_RS27010	95154..96122	-	969	WP_004225014.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..196000	196000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17689.52 Da
• Isoelectric Point: 9.2033

>T108219 WP_004902249.1 NZ_CP030924:90646-91128 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNKVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T108219 NZ_CP030924:90646-91128 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACAAAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT108219 WP_004902250.1 NZ_CP030924:90377-90655 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT108219 NZ_CP030924:90377-90655 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3