Detailed information of TA system TA108207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1703030..1703616
• Replicon: chromosome
• Accession: NZ_CP030923
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KC-Pl-HB1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7Z9B6M3
• Locus tag: DTN91_RS08475
• Protein ID: WP_029884514.1
• Coordinates: 1703248..1703616 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: DTN91_RS08470
• Protein ID: WP_004174006.1
• Coordinates: 1703030..1703251 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTN91_RS08450	1699187..1700113	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DTN91_RS08455	1700110..1701387	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
DTN91_RS08460	1701384..1702151	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DTN91_RS08465	1702153..1702866	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
DTN91_RS08470	1703030..1703251	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DTN91_RS08475	1703248..1703616	+	369	WP_029884514.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DTN91_RS08480	1703889..1705205	+	1317	WP_004901106.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
DTN91_RS08485	1705312..1706199	+	888	WP_004901104.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
DTN91_RS08490	1706196..1707041	+	846	WP_004901101.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
DTN91_RS08495	1707043..1708113	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1663144..1708850	45706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13583.01 Da
• Isoelectric Point: 8.6410

>T108207 WP_029884514.1 NZ_CP030923:1703248-1703616 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T108207 NZ_CP030923:1703248-1703616 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTATGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT108207 WP_004174006.1 NZ_CP030923:1703030-1703251 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT108207 NZ_CP030923:1703030-1703251 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A7Z9B6M3

Antitoxin

• AlphaFold DB: A0A5E5YJY7