Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 24557..25082 Replicon plasmid pKL53-L
Accession NZ_CP030920
Organism Escherichia coli strain KL53

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag CPT07_RS25345 Protein ID WP_016240353.1
Coordinates 24777..25082 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A8H9ZZC6
Locus tag CPT07_RS25340 Protein ID WP_016240352.1
Coordinates 24557..24775 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPT07_RS25310 21846..22946 + 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
CPT07_RS25315 23027..23278 - 252 WP_105966327.1 hypothetical protein -
CPT07_RS25320 23314..23730 - 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin -
CPT07_RS25325 23727..23957 - 231 WP_001261283.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CPT07_RS25340 24557..24775 + 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CPT07_RS25345 24777..25082 + 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB Toxin
CPT07_RS25350 25252..26229 + 978 Protein_30 hypothetical protein -
CPT07_RS25355 26465..27259 + 795 WP_016240356.1 site-specific integrase -
CPT07_RS25360 27836..28084 + 249 Protein_32 transposase -
CPT07_RS25365 28149..28376 + 228 Protein_33 hypothetical protein -
CPT07_RS25370 28429..29657 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..259094 259094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11518.20 Da        Isoelectric Point: 5.6752

>T108196 WP_016240353.1 NZ_CP030920:24777-25082 [Escherichia coli]
MQFKVYAYKRESCYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T108196 NZ_CP030920:24777-25082 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGTTGCTATCGTCTGTTCGTGGATGTACAGAGTGACATCATCGATAC
CCCCGGGCGGCGCATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTTTCGGATAAGGTCTCCCGTGATCTCTATCCGGTGG
TGCACATCGGGGACGACAGCTATCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCTGTCACCGGGGAGGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8321.25 Da        Isoelectric Point: 4.5358

>AT108196 WP_016240352.1 NZ_CP030920:24557-24775 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Download         Length: 219 bp

>AT108196 NZ_CP030920:24557-24775 [Escherichia coli]
ATGAAGCAGCGCATCACCGTCACAATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATATCCGGTCT
GGTGAACACGGCCATGCAGAATGAGGCCCGCCGTTTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGAAGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References