Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23314..23957 Replicon plasmid pKL53-L
Accession NZ_CP030920
Organism Escherichia coli strain KL53

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag CPT07_RS25320 Protein ID WP_032192044.1
Coordinates 23314..23730 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag CPT07_RS25325 Protein ID WP_001261283.1
Coordinates 23727..23957 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPT07_RS25300 19181..20524 + 1344 WP_032192047.1 APC family permease -
CPT07_RS25310 21846..22946 + 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
CPT07_RS25315 23027..23278 - 252 WP_105966327.1 hypothetical protein -
CPT07_RS25320 23314..23730 - 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
CPT07_RS25325 23727..23957 - 231 WP_001261283.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CPT07_RS25340 24557..24775 + 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
CPT07_RS25345 24777..25082 + 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
CPT07_RS25350 25252..26229 + 978 Protein_30 hypothetical protein -
CPT07_RS25355 26465..27259 + 795 WP_016240356.1 site-specific integrase -
CPT07_RS25360 27836..28084 + 249 Protein_32 transposase -
CPT07_RS25365 28149..28376 + 228 Protein_33 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..259094 259094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T108195 WP_032192044.1 NZ_CP030920:c23730-23314 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T108195 NZ_CP030920:c23730-23314 [Escherichia coli]
ATGAAGAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGACGCGCCT
GGAGCAGGCAGTACTGCGCGGCCATCGTATCGTGATCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCACCGCGCCACGTCCAGCTGGTTGACGCGTTCTGCGCACGCCTCGACGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGATGCGACCATTGAGATCAAGGTCGCACTGCGCCTCGCCGGCACGCCGATCGGACCGAACGACACGGCAATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTGCTGGTGACGAATAATACACGGGAGTTTGAGCGGGTGCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT108195 WP_001261283.1 NZ_CP030920:c23957-23727 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT108195 NZ_CP030920:c23957-23727 [Escherichia coli]
ATGCGCACAGTTTCTATTTTCAAAAACGGCAACAACCGCGCCATTCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCAACCTGGGGTTCGTTCGCGCAGCTCG
AAAAAGCTGACCCTGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGCTTTGAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References