Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2906931..2907585 Replicon chromosome
Accession NZ_CP030919
Organism Escherichia coli strain KL53

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CPT07_RS15270 Protein ID WP_000244781.1
Coordinates 2906931..2907338 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CPT07_RS15275 Protein ID WP_000354046.1
Coordinates 2907319..2907585 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPT07_RS15250 2902888..2904621 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
CPT07_RS15255 2904627..2905337 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CPT07_RS15260 2905362..2906258 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CPT07_RS15265 2906370..2906891 + 522 WP_044863754.1 flavodoxin FldB -
CPT07_RS15270 2906931..2907338 - 408 WP_000244781.1 protein YgfX Toxin
CPT07_RS15275 2907319..2907585 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CPT07_RS15280 2907828..2908808 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
CPT07_RS15285 2908885..2909544 - 660 WP_000250274.1 hemolysin III family protein -
CPT07_RS15290 2909708..2910019 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CPT07_RS15295 2910064..2911497 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
CPT07_RS15300 2911554..2912297 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T108190 WP_000244781.1 NZ_CP030919:c2907338-2906931 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T108190 NZ_CP030919:c2907338-2906931 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT108190 WP_000354046.1 NZ_CP030919:c2907585-2907319 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT108190 NZ_CP030919:c2907585-2907319 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References