Detailed information of TA system TA108185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1428718..1429244
• Replicon: chromosome
• Accession: NZ_CP030919
• Organism: Escherichia coli strain KL53

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CPT07_RS07690
• Protein ID: WP_000323025.1
• Coordinates: 1428957..1429244 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CPT07_RS07685
• Protein ID: WP_000534858.1
• Coordinates: 1428718..1428957 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT07_RS07650	1424080..1424436	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
CPT07_RS28020	1425047..1425334	+	288	Protein_1388	hypothetical protein	-
CPT07_RS07665	1426103..1427416	+	1314	WP_000589005.1	ISNCY family transposase	-
CPT07_RS07675	1427853..1428185	-	333	WP_001301033.1	protein FlxA	-
CPT07_RS07680	1428388..1428693	-	306	WP_001326990.1	hypothetical protein	-
CPT07_RS07685	1428718..1428957	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CPT07_RS07690	1428957..1429244	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CPT07_RS07695	1429316..1429471	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CPT07_RS07700	1429688..1429939	+	252	WP_097360105.1	hypothetical protein	-
CPT07_RS07705	1430006..1430284	+	279	WP_012304870.1	hypothetical protein	-
CPT07_RS07710	1430286..1430897	+	612	Protein_1397	DUF968 domain-containing protein	-
CPT07_RS07720	1432208..1432648	+	441	Protein_1399	DUF968 domain-containing protein	-
CPT07_RS07725	1432662..1433414	+	753	WP_001047135.1	antitermination protein	-
CPT07_RS27790	1433692..1433781	-	90	WP_120795389.1	hypothetical protein	-
CPT07_RS07730	1433836..1434048	-	213	WP_000087756.1	cold shock-like protein CspF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1405513..1449208	43695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T108185 WP_000323025.1 NZ_CP030919:1428957-1429244 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T108185 NZ_CP030919:1428957-1429244 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT108185 WP_000534858.1 NZ_CP030919:1428718-1428957 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT108185 NZ_CP030919:1428718-1428957 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8