Detailed information of TA system TA108175

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 12851..13530
• Replicon: chromosome
• Accession: NZ_CP030919
• Organism: Escherichia coli strain KL53

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: CPT07_RS00100
• Protein ID: WP_000854680.1
• Coordinates: 12851..13192 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: CPT07_RS00105
• Protein ID: WP_000070396.1
• Coordinates: 13213..13530 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT07_RS00080	8389..8586	+	198	WP_033801633.1	hypothetical protein	-
CPT07_RS00085	8579..10444	+	1866	WP_045148748.1	cell envelope integrity protein TolA	-
CPT07_RS00090	10783..11061	+	279	WP_033801635.1	hypothetical protein	-
CPT07_RS00095	11097..12311	-	1215	WP_033801639.1	tyrosine-type recombinase/integrase	-
CPT07_RS00100	12851..13192	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
CPT07_RS00105	13213..13530	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CPT07_RS00110	13549..13770	-	222	WP_000691994.1	DUF987 domain-containing protein	-
CPT07_RS00115	13779..14255	-	477	WP_000811693.1	RadC family protein	-
CPT07_RS00120	14271..14729	-	459	WP_000211838.1	antirestriction protein	-
CPT07_RS00125	14827..15066	-	240	WP_000194654.1	DUF905 domain-containing protein	-
CPT07_RS00130	15143..15610	-	468	WP_057107658.1	hypothetical protein	-
CPT07_RS00135	15634..16077	-	444	WP_016239438.1	phage transcriptional regulator	-
CPT07_RS00140	16077..16313	-	237	WP_057107659.1	hypothetical protein	-
CPT07_RS00145	16354..17055	-	702	WP_023326104.1	WYL domain-containing protein	-
CPT07_RS00150	17272..18093	-	822	WP_085381467.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	356..18093	17737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T108175 WP_000854680.1 NZ_CP030919:c13192-12851 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T108175 NZ_CP030919:c13192-12851 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT108175 WP_000070396.1 NZ_CP030919:c13530-13213 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT108175 NZ_CP030919:c13530-13213 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7