Detailed information of TA system TA108173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 36538..37124
• Replicon: plasmid pY89
• Accession: NZ_CP030914
• Organism: Pseudomonas aeruginosa strain Y89

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: Y89_RS33080
• Protein ID: WP_003120987.1
• Coordinates: 36825..37124 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: Y89_RS33075
• Protein ID: WP_003448662.1
• Coordinates: 36538..36828 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y89_RS33060	32132..34009	+	1878	WP_023911870.1	hypothetical protein	-
Y89_RS33065	34006..35982	+	1977	WP_031275715.1	DEAD/DEAH box helicase	-
Y89_RS33070	36123..36467	+	345	WP_003448665.1	hypothetical protein	-
Y89_RS33075	36538..36828	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
Y89_RS33080	36825..37124	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y89_RS33085	37325..38449	+	1125	WP_023435880.1	TcpQ domain-containing protein	-
Y89_RS33090	38449..40158	+	1710	WP_023435879.1	PilN family type IVB pilus formation outer membrane protein	-
Y89_RS33095	40162..41487	+	1326	WP_023435878.1	type 4b pilus protein PilO2	-
Y89_RS33100	41477..42010	+	534	WP_023435877.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85842	85842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T108173 WP_003120987.1 NZ_CP030914:c37124-36825 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T108173 NZ_CP030914:c37124-36825 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT108173 WP_003448662.1 NZ_CP030914:c36828-36538 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT108173 NZ_CP030914:c36828-36538 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73