Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5517677..5518291 Replicon chromosome
Accession NZ_CP030912
Organism Pseudomonas aeruginosa strain Y82

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag Y82_RS26800 Protein ID WP_071534354.1
Coordinates 5518109..5518291 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag Y82_RS26795 Protein ID WP_012077229.1
Coordinates 5517677..5518081 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y82_RS26770 5513817..5514527 + 711 WP_012074129.1 hypothetical protein -
Y82_RS26775 5514524..5515444 + 921 WP_012074128.1 hypothetical protein -
Y82_RS26780 5515441..5515980 + 540 WP_012074127.1 hypothetical protein -
Y82_RS26785 5515980..5516678 + 699 WP_033896049.1 hypothetical protein -
Y82_RS26790 5516663..5517634 + 972 WP_012077228.1 hypothetical protein -
Y82_RS26795 5517677..5518081 - 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
Y82_RS26800 5518109..5518291 - 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
Y82_RS26805 5518834..5519766 - 933 WP_003120918.1 ZIP family metal transporter -
Y82_RS26810 5519785..5520396 - 612 WP_003098862.1 superoxide dismutase -
Y82_RS26815 5520409..5520858 - 450 WP_003094369.1 hypothetical protein -
Y82_RS26820 5520886..5522262 - 1377 WP_003098863.1 class II fumarate hydratase -
Y82_RS26825 5522255..5522650 - 396 WP_003162782.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5480800..5518291 37491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T108161 WP_071534354.1 NZ_CP030912:c5518291-5518109 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T108161 NZ_CP030912:c5518291-5518109 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT108161 WP_012077229.1 NZ_CP030912:c5518081-5517677 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT108161 NZ_CP030912:c5518081-5517677 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References