Detailed information of TA system TA108158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2884951..2885993
• Replicon: chromosome
• Accession: NZ_CP030912
• Organism: Pseudomonas aeruginosa strain Y82

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: Y82_RS14150
• Protein ID: WP_003153636.1
• Coordinates: 2885418..2885993 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: Y82_RS14145
• Protein ID: WP_003050245.1
• Coordinates: 2884951..2885421 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y82_RS14110	2880343..2881761	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
Y82_RS14115	2881751..2882662	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
Y82_RS14120	2882659..2883351	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
Y82_RS14125	2883348..2883746	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
Y82_RS14130	2883758..2884117	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
Y82_RS14135	2884134..2884367	-	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
Y82_RS14140	2884364..2884747	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
Y82_RS14145	2884951..2885421	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
Y82_RS14150	2885418..2885993	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
Y82_RS14155	2886011..2886925	+	915	WP_016852809.1	AAA family ATPase	-
Y82_RS14160	2886922..2887392	+	471	WP_003090160.1	hypothetical protein	-
Y82_RS14165	2887389..2887889	+	501	WP_003090159.1	hypothetical protein	-
Y82_RS14170	2887889..2888791	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
Y82_RS14175	2888830..2889555	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T108158 WP_003153636.1 NZ_CP030912:2885418-2885993 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T108158 NZ_CP030912:2885418-2885993 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT108158 WP_003050245.1 NZ_CP030912:2884951-2885421 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT108158 NZ_CP030912:2884951-2885421 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68