Detailed information of TA system TA108153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5579547..5580133
• Replicon: chromosome
• Accession: NZ_CP030911
• Organism: Pseudomonas aeruginosa strain Y71

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: Ysp71_RS26910
• Protein ID: WP_003120987.1
• Coordinates: 5579834..5580133 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: Ysp71_RS26905
• Protein ID: WP_003448662.1
• Coordinates: 5579547..5579837 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ysp71_RS26890	5575141..5577018	+	1878	WP_023911870.1	hypothetical protein	-
Ysp71_RS26895	5577015..5578991	+	1977	WP_031275715.1	DEAD/DEAH box helicase	-
Ysp71_RS26900	5579132..5579476	+	345	WP_003448665.1	hypothetical protein	-
Ysp71_RS26905	5579547..5579837	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
Ysp71_RS26910	5579834..5580133	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Ysp71_RS26915	5580334..5581458	+	1125	WP_023435880.1	TcpQ domain-containing protein	-
Ysp71_RS26920	5581458..5583167	+	1710	WP_023435879.1	PilN family type IVB pilus formation outer membrane protein	-
Ysp71_RS26925	5583171..5584496	+	1326	WP_023435878.1	type 4b pilus protein PilO2	-
Ysp71_RS26930	5584486..5585019	+	534	WP_023435877.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5555242..5641369	86127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T108153 WP_003120987.1 NZ_CP030911:c5580133-5579834 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T108153 NZ_CP030911:c5580133-5579834 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT108153 WP_003448662.1 NZ_CP030911:c5579837-5579547 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT108153 NZ_CP030911:c5579837-5579547 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73