Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 654779..655393 Replicon chromosome
Accession NZ_CP030911
Organism Pseudomonas aeruginosa strain Y71

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag Ysp71_RS03105 Protein ID WP_071534354.1
Coordinates 654779..654961 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag Ysp71_RS03110 Protein ID WP_012077229.1
Coordinates 654989..655393 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Ysp71_RS03085 650647..650935 - 289 Protein_602 DDE-type integrase/transposase/recombinase -
Ysp71_RS03090 651001..652377 + 1377 WP_076611796.1 IS30-like element ISPa37 family transposase -
Ysp71_RS03095 652385..653261 - 877 Protein_604 IS3 family transposase -
Ysp71_RS03100 653326..654190 - 865 Protein_605 site-specific integrase -
Ysp71_RS03105 654779..654961 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
Ysp71_RS03110 654989..655393 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
Ysp71_RS03115 655436..656407 - 972 WP_012077228.1 hypothetical protein -
Ysp71_RS03120 656392..657090 - 699 WP_033896049.1 hypothetical protein -
Ysp71_RS03125 657090..657629 - 540 WP_012074127.1 hypothetical protein -
Ysp71_RS03130 657626..658546 - 921 WP_012074128.1 hypothetical protein -
Ysp71_RS03135 658543..659253 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 650705..708965 58260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T108147 WP_071534354.1 NZ_CP030911:654779-654961 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T108147 NZ_CP030911:654779-654961 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT108147 WP_012077229.1 NZ_CP030911:654989-655393 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT108147 NZ_CP030911:654989-655393 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References