Detailed information of TA system TA108146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 153417..153922
• Replicon: chromosome
• Accession: NZ_CP030911
• Organism: Pseudomonas aeruginosa strain Y71

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: Ysp71_RS00690
• Protein ID: WP_003083773.1
• Coordinates: 153417..153698 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: Ysp71_RS00695
• Protein ID: WP_003083775.1
• Coordinates: 153695..153922 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ysp71_RS00665	148668..150017	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
Ysp71_RS00670	150066..150752	+	687	WP_003083762.1	FadR family transcriptional regulator	-
Ysp71_RS00675	150853..151587	+	735	WP_023081667.1	GntR family transcriptional regulator	-
Ysp71_RS00680	151791..152177	+	387	WP_014602345.1	aegerolysin family protein	-
Ysp71_RS00685	152209..153117	-	909	WP_003083769.1	LysR family transcriptional regulator	-
Ysp71_RS00690	153417..153698	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
Ysp71_RS00695	153695..153922	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
Ysp71_RS00700	154098..154718	-	621	WP_003101226.1	hypothetical protein	-
Ysp71_RS00705	154819..155319	+	501	WP_003101228.1	LEA type 2 family protein	-
Ysp71_RS00710	155392..155733	+	342	WP_023081666.1	alkylphosphonate utilization protein	-
Ysp71_RS00715	155833..156417	-	585	WP_073674849.1	DUF4276 family protein	-
Ysp71_RS00720	156414..157622	-	1209	WP_023081665.1	AAA family ATPase	-
Ysp71_RS00725	158014..158127	+	114	Protein_144	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	158014..158130	116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T108146 WP_003083773.1 NZ_CP030911:c153698-153417 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T108146 NZ_CP030911:c153698-153417 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATTGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT108146 WP_003083775.1 NZ_CP030911:c153922-153695 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT108146 NZ_CP030911:c153922-153695 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9