Detailed information of TA system TA108140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1252700..1253340
• Replicon: chromosome
• Accession: NZ_CP030910
• Organism: Pseudomonas aeruginosa strain Y31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Y31_RS06110
• Protein ID: WP_003105740.1
• Coordinates: 1252930..1253340 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: Y31_RS06105
• Protein ID: WP_003158175.1
• Coordinates: 1252700..1252930 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y31_RS06090	1247898..1249787	-	1890	WP_003105732.1	hypothetical protein	-
Y31_RS06095	1250008..1250217	-	210	WP_003105733.1	cold-shock protein	-
Y31_RS06100	1250525..1252444	-	1920	WP_004352838.1	type I DNA topoisomerase	-
Y31_RS06105	1252700..1252930	+	231	WP_003158175.1	antitoxin	Antitoxin
Y31_RS06110	1252930..1253340	+	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
Y31_RS06115	1253362..1253622	+	261	WP_003105742.1	hypothetical protein	-
Y31_RS06125	1253822..1254040	+	219	WP_003105747.1	hypothetical protein	-
Y31_RS06130	1254106..1254351	-	246	WP_023102333.1	CrpP family protein	-
Y31_RS06135	1254459..1254947	-	489	WP_003105748.1	single-stranded DNA-binding protein	-
Y31_RS06140	1254977..1255816	-	840	WP_003105750.1	Rha family transcriptional regulator	-
Y31_RS06145	1255863..1256411	-	549	WP_003105753.1	DUF3158 family protein	-
Y31_RS06150	1256417..1257145	-	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
Y31_RS06155	1257301..1257498	-	198	WP_003105756.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1166409..1272911	106502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T108140 WP_003105740.1 NZ_CP030910:1252930-1253340 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T108140 NZ_CP030910:1252930-1253340 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT108140 WP_003158175.1 NZ_CP030910:1252700-1252930 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT108140 NZ_CP030910:1252700-1252930 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2