Detailed information of TA system TA108135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4705245..4705821
• Replicon: chromosome
• Accession: NZ_CP030874
• Organism: Raoultella sp. X13

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: DSD31_RS22440
• Protein ID: WP_032716505.1
• Coordinates: 4705534..4705821 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: DSD31_RS22435
• Protein ID: WP_113991318.1
• Coordinates: 4705245..4705547 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSD31_RS22420	4701189..4701524	+	336	WP_088883471.1	endoribonuclease SymE	-
DSD31_RS22425	4701755..4703827	+	2073	WP_104895474.1	AAA family ATPase	-
DSD31_RS22430	4703827..4705149	+	1323	WP_113991317.1	restriction endonuclease	-
DSD31_RS22435	4705245..4705547	-	303	WP_113991318.1	BrnA antitoxin family protein	Antitoxin
DSD31_RS22440	4705534..4705821	-	288	WP_032716505.1	BrnT family toxin	Toxin
DSD31_RS22445	4705938..4706357	-	420	WP_004857515.1	FosA family fosfomycin resistance glutathione transferase	-
DSD31_RS22450	4706361..4707260	-	900	WP_113991319.1	LysR family transcriptional regulator	-
DSD31_RS22455	4707348..4708130	+	783	WP_113991320.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T108135 WP_032716505.1 NZ_CP030874:c4705821-4705534 [Raoultella sp. X13]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T108135 NZ_CP030874:c4705821-4705534 [Raoultella sp. X13]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11539.05 Da
• Isoelectric Point: 7.0165

>AT108135 WP_113991318.1 NZ_CP030874:c4705547-4705245 [Raoultella sp. X13]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLSEK

Nucleotide

• Length: 303 bp

>AT108135 NZ_CP030874:c4705547-4705245 [Raoultella sp. X13]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAACGCGATTTTAAGAGAAGCGATGATTCGCGATCTGAGCGAGAAATAA

Structures

Toxin

• AlphaFold DB: A0A808YBH6