Detailed information of TA system TA108132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2689151..2689902
• Replicon: chromosome
• Accession: NZ_CP030874
• Organism: Raoultella sp. X13

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: DSD31_RS12850
• Protein ID: WP_113991085.1
• Coordinates: 2689420..2689902 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: DSD31_RS12845
• Protein ID: WP_015584326.1
• Coordinates: 2689151..2689429 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSD31_RS12835	2685929..2686426	+	498	WP_048023692.1	hypothetical protein	-
DSD31_RS12840	2686584..2688509	-	1926	WP_113991393.1	C1 family peptidase	-
DSD31_RS12845	2689151..2689429	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
DSD31_RS12850	2689420..2689902	+	483	WP_113991085.1	GNAT family N-acetyltransferase	Toxin
DSD31_RS12865	2690978..2691844	-	867	Protein_2460	ATP-binding protein	-
DSD31_RS12870	2691935..2692090	-	156	Protein_2461	transposase	-
DSD31_RS12875	2692189..2692410	+	222	WP_162786319.1	hypothetical protein	-
DSD31_RS12880	2692686..2693198	+	513	WP_047417481.1	hypothetical protein	-
DSD31_RS12885	2693201..2693752	+	552	WP_048023574.1	YfbU family protein	-
DSD31_RS12890	2693831..2694328	-	498	WP_113991087.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2686584..2699585	13001
	-	flank	IS/Tn	-	-	2691863..2692090	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17686.55 Da
• Isoelectric Point: 9.9460

>T108132 WP_113991085.1 NZ_CP030874:2689420-2689902 [Raoultella sp. X13]
MGMRPPEPLSTGHNIAEFFCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T108132 NZ_CP030874:2689420-2689902 [Raoultella sp. X13]
ATGGGAATGAGACCACCGGAACCACTCAGCACTGGACACAACATAGCAGAATTTTTTTGTCAGGATCAGGTTCTTAACGA
GTGGTTGAAGAAGAAGGCGCTGAAGAACCACAGTACAGGCATTTCAAGGGTCTTTGTGGTCTGTGCCGAAAACACAAAAA
GGGTCATCGCGTATTACAGCTTATCATCCGGGAGCGTGCACAGAAACACAGTACCGGGGGCATACCGTCGTAATGCACCT
GAAGCTGTACCGGTCATTGTTCTGGGAAGGCTTGCGGTCGATGCAGCCTGGGCAGGGAAGGGACTCGGAGCGGCACTGCT
TAAGGATGCAATATACCGTACAGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTCCATGCCTTGAATGATG
AAGTCAGGGAGTTTTACACCAGGTTTGGATTCGAGCCCTCGATTATCAATACACTGACATTATTGTTCCCGATAAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT108132 WP_015584326.1 NZ_CP030874:2689151-2689429 [Raoultella sp. X13]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT108132 NZ_CP030874:2689151-2689429 [Raoultella sp. X13]
ATGAAACCAGACGTTCGTGAAGCTCCGATAAACATTCGGGCGAAAACATCCCAGCGCGACCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTTGAAGTGGCCTGTCGCGAAGCTCAGGACATTCTGTTGGATCAGCGCT
TGTTCCTGCTGGACGATAAGCAGTTTGCTAGTTTCATGGAAGAGCTTGACGCACCTATGACAGCGGAGCGTCAGGCACGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8