Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 74694..75241 Replicon chromosome
Accession NZ_CP030874
Organism Raoultella sp. X13

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag DSD31_RS00355 Protein ID WP_048025274.1
Coordinates 74694..75002 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag DSD31_RS00360 Protein ID WP_004869059.1
Coordinates 75005..75241 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSD31_RS00325 69882..70622 + 741 WP_015585491.1 4-oxalmesaconate hydratase -
DSD31_RS00330 70619..71332 + 714 WP_015585492.1 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase -
DSD31_RS00335 71337..72428 + 1092 WP_015585493.1 4-oxalomesaconate tautomerase -
DSD31_RS00340 72425..73291 + 867 WP_015585494.1 NAD(P)-dependent oxidoreductase -
DSD31_RS00345 73292..73567 - 276 WP_162769256.1 hypothetical protein -
DSD31_RS00350 73676..74578 - 903 WP_015585496.1 EamA family transporter -
DSD31_RS00355 74694..75002 - 309 WP_048025274.1 CcdB family protein Toxin
DSD31_RS00360 75005..75241 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
DSD31_RS00365 75347..76780 - 1434 WP_069476354.1 PTS N-acetylmuramic acid transporter subunit IIBC -
DSD31_RS00370 76805..77707 - 903 WP_044350979.1 N-acetylmuramic acid 6-phosphate etherase -
DSD31_RS00375 77872..79035 - 1164 WP_004869055.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T108120 WP_048025274.1 NZ_CP030874:c75002-74694 [Raoultella sp. X13]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T108120 NZ_CP030874:c75002-74694 [Raoultella sp. X13]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGCCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAGCGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT108120 WP_004869059.1 NZ_CP030874:c75241-75005 [Raoultella sp. X13]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT108120 NZ_CP030874:c75241-75005 [Raoultella sp. X13]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References