Detailed information of TA system TA108115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 35353..35903
• Replicon: plasmid unnamed1
• Accession: NZ_CP030872
• Organism: Acetobacter sp. JWB

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7JII7
• Locus tag: DS739_RS13405
• Protein ID: WP_003618711.1
• Coordinates: 35353..35646 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D5QDQ9
• Locus tag: DS739_RS13410
• Protein ID: WP_003618686.1
• Coordinates: 35643..35903 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS739_RS13375	30454..30939	+	486	WP_082823248.1	helix-turn-helix domain-containing protein	-
DS739_RS13380	31064..32674	-	1611	WP_006115450.1	IS66 family transposase	-
DS739_RS13385	32738..33085	-	348	WP_012812355.1	IS66 family insertion sequence element accessory protein TnpB	-
DS739_RS13390	33082..33441	-	360	WP_012812356.1	transposase	-
DS739_RS13395	33540..33959	-	420	Protein_31	DUF1275 domain-containing protein	-
DS739_RS13400	33959..35221	-	1263	WP_006115449.1	sodium:proton antiporter	-
DS739_RS13405	35353..35646	-	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
DS739_RS13410	35643..35903	-	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DS739_RS13415	36496..37569	-	1074	WP_006115447.1	alkene reductase	-
DS739_RS13420	37769..38236	-	468	WP_050793094.1	hypothetical protein	-
DS739_RS13430	39224..39958	-	735	WP_039891505.1	class I SAM-dependent methyltransferase	-
DS739_RS13435	40026..40466	-	441	WP_039891503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137977	137977
	-	flank	IS/Tn	-	-	32738..33085	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11605.35 Da
• Isoelectric Point: 7.9998

>T108115 WP_003618711.1 NZ_CP030872:c35646-35353 [Acetobacter sp. JWB]
MKEYCLEFDDRALREWDALDGSVRKKFEKKLEKLIWNPHSPGNELHRDLSGFYKIKLLKDGYRLVYQVIDERIVIYVIAV
GRRADNEIYELASKRTE

Nucleotide

• Length: 294 bp

>T108115 NZ_CP030872:c35646-35353 [Acetobacter sp. JWB]
ATGAAAGAGTATTGTCTTGAATTCGACGACAGAGCACTCAGAGAGTGGGATGCTCTTGACGGCAGCGTGCGTAAGAAATT
TGAAAAGAAGCTGGAAAAGCTGATCTGGAACCCTCACTCTCCAGGAAACGAACTGCATCGCGACCTATCAGGGTTTTATA
AGATCAAGCTTCTGAAAGATGGTTACCGCCTTGTTTATCAGGTCATCGACGAACGGATCGTCATCTATGTAATTGCCGTT
GGCCGGAGAGCCGATAATGAGATTTATGAACTGGCTTCGAAGCGAACAGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9762.23 Da
• Isoelectric Point: 10.4645

>AT108115 WP_003618686.1 NZ_CP030872:c35903-35643 [Acetobacter sp. JWB]
MSAVTFRVDETLKAAAVKKLSAQGISLSDALRDTLAYIAETGRSPVKRRLVTDEDAELIEIVRERLKNPAQKHRMTFAEL
KNRHRE

Nucleotide

• Length: 261 bp

>AT108115 NZ_CP030872:c35903-35643 [Acetobacter sp. JWB]
ATGTCTGCTGTTACATTCCGGGTAGATGAAACGCTGAAGGCAGCCGCAGTTAAGAAGTTGTCGGCTCAAGGCATTTCACT
TTCTGACGCACTGCGCGATACGCTCGCGTATATTGCTGAAACGGGTCGCTCTCCCGTCAAGCGGAGGCTTGTCACAGACG
AGGATGCGGAGCTTATCGAGATTGTGCGCGAACGCCTAAAAAACCCTGCCCAAAAGCATCGTATGACTTTTGCAGAGCTT
AAGAACCGTCATCGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T9M6