Detailed information of TA system TA108113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2292106..2292689
• Replicon: chromosome
• Accession: NZ_CP030871
• Organism: Acetobacter sp. JWB

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F1YSB1
• Locus tag: DS739_RS10860
• Protein ID: WP_006115878.1
• Coordinates: 2292106..2292450 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F1YSB0
• Locus tag: DS739_RS10865
• Protein ID: WP_006115877.1
• Coordinates: 2292447..2292689 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS739_RS10835	2287557..2288639	-	1083	WP_170315297.1	IS5 family transposase	-
DS739_RS10840	2288716..2288886	+	171	Protein_2082	integrase	-
DS739_RS10845	2289023..2290114	-	1092	WP_035352416.1	IS5 family transposase	-
DS739_RS10850	2290193..2291275	+	1083	Protein_2084	site-specific integrase	-
DS739_RS10855	2291583..2291906	-	324	WP_139018863.1	hypothetical protein	-
DS739_RS10860	2292106..2292450	-	345	WP_006115878.1	endoribonuclease MazF	Toxin
DS739_RS10865	2292447..2292689	-	243	WP_006115877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DS739_RS10870	2292996..2294387	+	1392	WP_035352412.1	site-specific integrase	-
DS739_RS10875	2294697..2295203	-	507	WP_139018862.1	hypothetical protein	-
DS739_RS10880	2295882..2296103	+	222	WP_006115875.1	hypothetical protein	-
DS739_RS10885	2296208..2296831	+	624	WP_035352402.1	hypothetical protein	-
DS739_RS10890	2297249..2297452	+	204	WP_035352404.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2261228..2297162	35934
	-	flank	IS/Tn	-	-	2289023..2290114	1091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (13-112)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12544.68 Da
• Isoelectric Point: 10.4109

>T108113 WP_006115878.1 NZ_CP030871:c2292450-2292106 [Acetobacter sp. JWB]
VSAKGTAVWVPDRGDIVWLEFDPQTGREQAGHRPAIVLSPASYNAKAGMMLCCPTTTKIKGYPFEVALTGKTASVVLSDQ
LRSLDWRVRKAKLKGKVLPEELEAIRQRVRLLVG

Nucleotide

• Length: 345 bp

>T108113 NZ_CP030871:c2292450-2292106 [Acetobacter sp. JWB]
GTGAGTGCCAAAGGAACGGCTGTCTGGGTTCCTGATCGCGGGGACATTGTCTGGCTTGAGTTTGATCCGCAGACGGGCAG
GGAACAGGCCGGGCACCGGCCTGCTATTGTGCTGAGTCCTGCCAGTTATAACGCTAAAGCCGGAATGATGCTGTGCTGCC
CAACAACGACCAAAATCAAAGGCTACCCCTTTGAAGTGGCTCTAACTGGAAAAACTGCCAGTGTGGTTTTGTCAGACCAG
CTTAGAAGTCTGGATTGGCGCGTGAGAAAGGCAAAGCTCAAAGGAAAAGTTTTACCAGAAGAGCTTGAGGCCATCCGGCA
GCGGGTCAGATTGCTGGTCGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8736.96 Da
• Isoelectric Point: 4.3094

>AT108113 WP_006115877.1 NZ_CP030871:c2292689-2292447 [Acetobacter sp. JWB]
MQGVVRKWGNSAAIRLPAGVLEAVSLKIDQAVDVREEDGRIIIEPVKTVPLKLEELVSGITAENRYDEMDFGPSVGGESW

Nucleotide

• Length: 243 bp

>AT108113 NZ_CP030871:c2292689-2292447 [Acetobacter sp. JWB]
ATGCAGGGCGTTGTCAGGAAATGGGGAAACAGTGCAGCCATTCGTCTTCCTGCTGGTGTGCTTGAAGCCGTCAGTCTGAA
GATCGATCAGGCTGTTGATGTGCGGGAAGAAGATGGGCGCATTATCATTGAGCCAGTCAAAACAGTTCCTCTCAAACTGG
AAGAATTAGTGTCAGGCATTACGGCCGAGAACCGCTATGATGAAATGGACTTTGGTCCGTCTGTGGGTGGGGAAAGCTGG
TGA

Structures

Toxin

• AlphaFold DB: F1YSB1

Antitoxin

• AlphaFold DB: F1YSB0