Detailed information of TA system TA108111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 709841..710396
• Replicon: chromosome
• Accession: NZ_CP030871
• Organism: Acetobacter sp. JWB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: F1YWD7
• Locus tag: DS739_RS03410
• Protein ID: WP_006117339.1
• Coordinates: 710133..710396 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: DS739_RS03405
• Protein ID: WP_081461476.1
• Coordinates: 709841..710125 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS739_RS03375	704887..706479	+	1593	WP_006117346.1	hypothetical protein	-
DS739_RS03380	706479..707288	+	810	WP_035354364.1	hypothetical protein	-
DS739_RS03385	707322..707534	+	213	WP_003628621.1	hypothetical protein	-
DS739_RS03390	708121..708579	+	459	WP_035354359.1	lysozyme	-
DS739_RS14125	708622..708780	+	159	WP_162784330.1	hypothetical protein	-
DS739_RS03395	708845..709537	+	693	WP_006117341.1	hypothetical protein	-
DS739_RS03400	709615..709824	+	210	WP_035354356.1	hypothetical protein	-
DS739_RS03405	709841..710125	-	285	WP_081461476.1	BrnA antitoxin family protein	Antitoxin
DS739_RS03410	710133..710396	-	264	WP_006117339.1	BrnT family toxin	Toxin
DS739_RS03415	710721..711101	-	381	Protein_657	endonuclease/exonuclease/phosphatase family protein	-
DS739_RS03420	711034..711720	-	687	WP_006116364.1	carbonic anhydrase	-
DS739_RS03425	711881..712504	-	624	WP_006116363.1	NYN domain-containing protein	-
DS739_RS03430	712662..713174	+	513	WP_035354129.1	2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase	-
DS739_RS03435	713265..713636	+	372	WP_003622533.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	673353..710396	37043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-90)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10074.56 Da
• Isoelectric Point: 9.7308

>T108111 WP_006117339.1 NZ_CP030871:c710396-710133 [Acetobacter sp. JWB]
MRYDWDEAKRQSNIAKHGVDFADAEDFEWDVAFVMTSRSANEPRLVAIAPINLRLHVLVFSIETRIVRIISLRKANKREA
KLYAAQT

Nucleotide

• Length: 264 bp

>T108111 NZ_CP030871:c710396-710133 [Acetobacter sp. JWB]
ATGAGATACGATTGGGACGAAGCAAAGCGTCAAAGTAACATCGCCAAGCATGGCGTTGATTTTGCTGATGCTGAAGACTT
CGAATGGGATGTGGCTTTTGTCATGACAAGTCGTAGCGCAAACGAGCCCCGCCTTGTTGCTATTGCCCCAATCAATCTCC
GCCTGCATGTGCTGGTTTTCAGCATTGAAACACGCATTGTCCGCATCATCAGCCTACGAAAGGCCAACAAACGGGAGGCA
AAGCTTTATGCCGCGCAAACCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10284.66 Da
• Isoelectric Point: 4.7435

>AT108111 WP_081461476.1 NZ_CP030871:c710125-709841 [Acetobacter sp. JWB]
MPTDEEDALINQGILQDADNPELTDQDFLNAKPASVAVPALAAQRRVRGPQKAPTKKLVSIRLDPDLIDRLKQDGPGWQG
RVNDMLRQAIGLTS

Nucleotide

• Length: 285 bp

>AT108111 NZ_CP030871:c710125-709841 [Acetobacter sp. JWB]
ATGCCTACGGATGAAGAAGATGCCCTGATCAATCAGGGCATCTTGCAAGATGCAGATAACCCCGAGCTAACGGATCAAGA
TTTTCTCAATGCCAAACCTGCATCTGTAGCTGTGCCAGCATTAGCAGCACAGCGTAGAGTAAGAGGGCCACAAAAAGCTC
CCACAAAGAAGCTTGTATCGATTCGTCTTGACCCCGATTTGATTGACCGGCTGAAACAGGACGGCCCTGGTTGGCAAGGG
CGCGTTAACGATATGCTACGGCAAGCCATAGGCCTAACCAGTTAG

Structures

Toxin

• AlphaFold DB: A0A346DWK5