Detailed information of TA system TA108110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 6938..7493
• Replicon: chromosome
• Accession: NZ_CP030871
• Organism: Acetobacter sp. JWB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: F1YWD7
• Locus tag: DS739_RS00045
• Protein ID: WP_006117339.1
• Coordinates: 7230..7493 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: DS739_RS00040
• Protein ID: WP_081461476.1
• Coordinates: 6938..7222 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS739_RS00010	1984..3576	+	1593	WP_006117346.1	hypothetical protein	-
DS739_RS00015	3576..4385	+	810	WP_035354364.1	hypothetical protein	-
DS739_RS00020	4419..4631	+	213	WP_003628621.1	hypothetical protein	-
DS739_RS00025	5218..5676	+	459	WP_035354359.1	lysozyme	-
DS739_RS14095	5719..5877	+	159	WP_162784330.1	hypothetical protein	-
DS739_RS00030	5942..6634	+	693	WP_006117341.1	hypothetical protein	-
DS739_RS00035	6712..6921	+	210	WP_035354356.1	hypothetical protein	-
DS739_RS00040	6938..7222	-	285	WP_081461476.1	BrnA antitoxin family protein	Antitoxin
DS739_RS00045	7230..7493	-	264	WP_006117339.1	BrnT family toxin	Toxin
DS739_RS00055	7929..8513	-	585	WP_006116589.1	TIGR00730 family Rossman fold protein	-
DS739_RS00060	8562..9794	-	1233	WP_006116590.1	MFS transporter	-
DS739_RS00065	9891..10466	+	576	WP_006116591.1	NUDIX hydrolase	-
DS739_RS00070	10500..10952	-	453	WP_035353112.1	protoporphyrinogen oxidase HemJ	-
DS739_RS00075	10955..11656	-	702	WP_081461452.1	HAD family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-90)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10074.56 Da
• Isoelectric Point: 9.7308

>T108110 WP_006117339.1 NZ_CP030871:c7493-7230 [Acetobacter sp. JWB]
MRYDWDEAKRQSNIAKHGVDFADAEDFEWDVAFVMTSRSANEPRLVAIAPINLRLHVLVFSIETRIVRIISLRKANKREA
KLYAAQT

Nucleotide

• Length: 264 bp

>T108110 NZ_CP030871:c7493-7230 [Acetobacter sp. JWB]
ATGAGATACGATTGGGACGAAGCAAAGCGTCAAAGTAACATCGCCAAGCATGGCGTTGATTTTGCTGATGCTGAAGACTT
CGAATGGGATGTGGCTTTTGTCATGACAAGTCGTAGCGCAAACGAGCCCCGCCTTGTTGCTATTGCCCCAATCAATCTCC
GCCTGCATGTGCTGGTTTTCAGCATTGAAACACGCATTGTCCGCATCATCAGCCTACGAAAGGCCAACAAACGGGAGGCA
AAGCTTTATGCCGCGCAAACCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10284.66 Da
• Isoelectric Point: 4.7435

>AT108110 WP_081461476.1 NZ_CP030871:c7222-6938 [Acetobacter sp. JWB]
MPTDEEDALINQGILQDADNPELTDQDFLNAKPASVAVPALAAQRRVRGPQKAPTKKLVSIRLDPDLIDRLKQDGPGWQG
RVNDMLRQAIGLTS

Nucleotide

• Length: 285 bp

>AT108110 NZ_CP030871:c7222-6938 [Acetobacter sp. JWB]
ATGCCTACGGATGAAGAAGATGCCCTGATCAATCAGGGCATCTTGCAAGATGCAGATAACCCCGAGCTAACGGATCAAGA
TTTTCTCAATGCCAAACCTGCATCTGTAGCTGTGCCAGCATTAGCAGCACAGCGTAGAGTAAGAGGGCCACAAAAAGCTC
CCACAAAGAAGCTTGTATCGATTCGTCTTGACCCCGATTTGATTGACCGGCTGAAACAGGACGGCCCTGGTTGGCAAGGG
CGCGTTAACGATATGCTACGGCAAGCCATAGGCCTAACCAGTTAG

Structures

Toxin

• AlphaFold DB: A0A346DWK5