Detailed information of TA system TA108107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 246374..247005
• Replicon: chromosome
• Accession: NZ_CP030865
• Organism: Micromonospora sp. B006

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MicB006_RS01240
• Protein ID: WP_116503284.1
• Coordinates: 246374..246799 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W7W0D3
• Locus tag: MicB006_RS01245
• Protein ID: WP_030500628.1
• Coordinates: 246796..247005 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MicB006_RS01225	242886..243986	-	1101	WP_116511057.1	serine hydrolase	-
MicB006_RS01230	244080..245564	+	1485	WP_116503281.1	FAD-dependent oxidoreductase	-
MicB006_RS01235	245569..246333	-	765	WP_116503282.1	crotonase/enoyl-CoA hydratase family protein	-
MicB006_RS01240	246374..246799	-	426	WP_116503284.1	PIN domain nuclease	Toxin
MicB006_RS01245	246796..247005	-	210	WP_030500628.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MicB006_RS01250	247116..247967	+	852	WP_116503285.1	Rieske 2Fe-2S domain-containing protein	-
MicB006_RS01255	247969..248985	-	1017	WP_116503287.1	LysM peptidoglycan-binding domain-containing protein	-
MicB006_RS01260	249000..249431	-	432	WP_073826667.1	hypothetical protein	-
MicB006_RS01265	249428..249901	-	474	WP_073826664.1	pilus assembly protein TadE	-
MicB006_RS01270	249898..250302	-	405	WP_174532978.1	pilus assembly protein	-
MicB006_RS01275	250380..250589	-	210	WP_013283462.1	hypothetical protein	-
MicB006_RS01280	250615..251478	-	864	WP_116503289.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (6-35)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15803.08 Da
• Isoelectric Point: 5.6045

>T108107 WP_116503284.1 NZ_CP030865:c246799-246374 [Micromonospora sp. B006]
VKLADYLIDTSALVRLLRDPDVLARWEQQVTAGLLAVCPLVELEFLYTARSVADRTRLTEQLRAAFGWVVMPDRIYERAA
EIQGELTARGTHRSAGAVDLLIAATAEYHGLSLLHYDRDFDQVGAVTGQPMHWLTRPDTIK

Nucleotide

• Length: 426 bp

>T108107 NZ_CP030865:c246799-246374 [Micromonospora sp. B006]
GTGAAACTGGCCGACTACCTGATCGACACCAGCGCCCTCGTCCGGCTCCTGCGTGATCCCGACGTGCTGGCTCGCTGGGA
ACAACAGGTGACAGCGGGCCTGCTCGCCGTGTGCCCGCTTGTCGAGCTGGAATTCCTCTACACCGCCCGGTCGGTCGCCG
ACCGGACGCGCCTGACCGAGCAGCTCCGGGCCGCCTTCGGCTGGGTGGTCATGCCGGACCGGATCTACGAGCGCGCCGCC
GAGATCCAGGGCGAGCTGACCGCGCGGGGCACGCACCGCTCGGCGGGCGCCGTCGACCTGCTCATCGCCGCCACCGCCGA
ATACCACGGCCTCTCGCTGCTGCACTACGACCGGGACTTCGACCAGGTCGGCGCCGTCACCGGCCAGCCGATGCACTGGC
TGACCCGACCCGACACGATCAAATAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7465.38 Da
• Isoelectric Point: 4.7872

>AT108107 WP_030500628.1 NZ_CP030865:c247005-246796 [Micromonospora sp. B006]
MTKILVDVDDEALADAAEAFGTKTKKDTVNVALREGAARLRRARALAELAERGQAGDFDELLDKGAYRS

Nucleotide

• Length: 210 bp

>AT108107 NZ_CP030865:c247005-246796 [Micromonospora sp. B006]
ATGACCAAGATCCTGGTGGACGTCGACGACGAGGCGCTGGCGGATGCCGCCGAGGCGTTCGGCACGAAGACCAAGAAGGA
CACGGTGAACGTCGCTCTCCGCGAGGGCGCGGCGAGACTGCGCCGCGCCCGGGCGCTGGCGGAGCTGGCCGAACGCGGGC
AGGCCGGTGACTTCGACGAACTGCTCGACAAGGGCGCCTACCGCTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q5HSK6