Detailed information of TA system TA108097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1188725..1189395
• Replicon: chromosome
• Accession: NZ_CP030861
• Organism: Pseudomonas aeruginosa strain HS9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CWE28_RS05835
• Protein ID: WP_023114647.1
• Coordinates: 1188725..1189144 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CWE28_RS05840
• Protein ID: WP_023114648.1
• Coordinates: 1189141..1189395 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWE28_RS05805	1184678..1185136	+	459	WP_023114640.1	helix-turn-helix domain-containing protein	-
CWE28_RS05810	1185175..1185543	-	369	WP_023114641.1	conjugal transfer transcriptional regulator TraJ	-
CWE28_RS05815	1185546..1185830	-	285	WP_023114642.1	hypothetical protein	-
CWE28_RS05820	1185842..1186084	-	243	WP_023114643.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
CWE28_RS05825	1186153..1187625	-	1473	WP_023114644.1	P-type conjugative transfer protein TrbL	-
CWE28_RS33095	1187627..1187785	-	159	WP_023114645.1	hypothetical protein	-
CWE28_RS05830	1187799..1188569	-	771	WP_023114646.1	P-type conjugative transfer protein TrbJ	-
CWE28_RS05835	1188725..1189144	-	420	WP_023114647.1	type II toxin-antitoxin system VapC family toxin	Toxin
CWE28_RS05840	1189141..1189395	-	255	WP_023114648.1	Arc family DNA-binding protein	Antitoxin
CWE28_RS05845	1190690..1191667	-	978	WP_031637437.1	plasmid replication	-
CWE28_RS05850	1191648..1192541	-	894	WP_031637438.1	helicase RepA family protein	-
CWE28_RS05855	1192544..1192768	-	225	WP_023114650.1	AlpA family phage regulatory protein	-
CWE28_RS05860	1192853..1193854	+	1002	WP_031637439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1174243..1195458	21215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14925.16 Da
• Isoelectric Point: 5.1890

>T108097 WP_023114647.1 NZ_CP030861:c1189144-1188725 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVVETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T108097 NZ_CP030861:c1189144-1188725 [Pseudomonas aeruginosa]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAGCCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGTGGAAACCCTCTACCTGTCCAGCGTGACGCTGGCCGAGCTGCTGTTCGGCATCGGCGCGCTTCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAGCTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCCATTATGCCGAGCTTGCGGTGAAGGCCCGCACAGCCGGAAAGGGTTTCCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTTGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.87 Da
• Isoelectric Point: 6.4760

>AT108097 WP_023114648.1 NZ_CP030861:c1189395-1189141 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT108097 NZ_CP030861:c1189395-1189141 [Pseudomonas aeruginosa]
ATGGCAATCCTGACAGTGCGGAATGTGCCGGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTGCGCGAGATTCTGGAAAGTGCAGTGAAGTCGGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCGCGTAGGGCTGACAAACGACGACTTCGCAGTATTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA