108095

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 36463..37505 Replicon chromosome
Accession NZ_CP030861
Organism Pseudomonas aeruginosa strain HS9

Toxin (Protein)


Gene name PP_4152 Uniprot ID -
Locus tag CWE28_RS00210 Protein ID WP_003153636.1
Coordinates 36463..37038 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID I3TV68
Locus tag CWE28_RS00215 Protein ID WP_003050245.1
Coordinates 37035..37505 (-) Length 157 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWE28_RS00185 32901..33626 - 726 WP_003050273.1 CBASS effector endonuclease NucC -
CWE28_RS00190 33665..34567 - 903 WP_003090158.1 nucleotidyltransferase domain-containing protein -
CWE28_RS00195 34567..35067 - 501 WP_003090159.1 hypothetical protein -
CWE28_RS00200 35064..35534 - 471 WP_023114496.1 hypothetical protein -
CWE28_RS00205 35531..36445 - 915 WP_023114497.1 AAA family ATPase -
CWE28_RS00210 36463..37038 - 576 WP_003153636.1 PIN domain-containing protein Toxin
CWE28_RS00215 37035..37505 - 471 WP_003050245.1 helix-turn-helix domain-containing protein Antitoxin
CWE28_RS00220 37709..38092 + 384 WP_003090167.1 RAQPRD family integrative conjugative element protein -
CWE28_RS00225 38089..38322 + 234 WP_003090170.1 TIGR03758 family integrating conjugative element protein -
CWE28_RS00230 38339..38698 + 360 WP_003090173.1 TIGR03745 family integrating conjugative element membrane protein -
CWE28_RS00235 38710..39108 + 399 WP_003050133.1 TIGR03750 family conjugal transfer protein -
CWE28_RS00240 39105..39797 + 693 WP_003090182.1 TIGR03746 family integrating conjugative element protein -
CWE28_RS00245 39794..40705 + 912 WP_006226028.1 TIGR03749 family integrating conjugative element protein -
CWE28_RS00250 40695..42113 + 1419 WP_006226029.1 TIGR03752 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-114)

Antitoxin

Helix-turn-helix domain, group 17

(85-133)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21629.78 Da        Isoelectric Point: 5.9995

>T108095 WP_003153636.1 NZ_CP030861:c37038-36463 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Download         Length: 576 bp

>T108095 NZ_CP030861:c37038-36463 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin


Download         Length: 157 a.a.        Molecular weight: 17245.62 Da        Isoelectric Point: 6.3803

>AT108095 WP_003050245.1 NZ_CP030861:c37505-37035 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Download         Length: 471 bp

>AT108095 NZ_CP030861:c37505-37035 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I3TV68

References