Detailed information of TA system TA108091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 1697232..1697828
• Replicon: chromosome
• Accession: NZ_CP030849
• Organism: Pandoraea sp. XY-2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: DRB87_RS07740
• Protein ID: WP_130025490.1
• Coordinates: 1697562..1697828 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: DRB87_RS07735
• Protein ID: WP_130025489.1
• Coordinates: 1697232..1697552 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB87_RS07705	1692372..1693130	+	759	WP_165385258.1	SDR family oxidoreductase	-
DRB87_RS07710	1693165..1694334	+	1170	WP_130025484.1	acetyl-CoA acetyltransferase	-
DRB87_RS07715	1694331..1695953	+	1623	WP_130025485.1	AMP-binding protein	-
DRB87_RS07720	1696049..1696282	+	234	WP_130025486.1	DUF4172 domain-containing protein	-
DRB87_RS07725	1696255..1696671	+	417	WP_130025487.1	DUF2726 domain-containing protein	-
DRB87_RS07730	1696740..1697048	-	309	WP_130025488.1	hypothetical protein	-
DRB87_RS07735	1697232..1697552	-	321	WP_130025489.1	BrnA antitoxin family protein	Antitoxin
DRB87_RS07740	1697562..1697828	-	267	WP_130025490.1	BrnT family toxin	Toxin
DRB87_RS07745	1698156..1699370	-	1215	WP_130025491.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (18-98)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10196.55 Da
• Isoelectric Point: 4.7722

>T108091 WP_130025490.1 NZ_CP030849:c1697828-1697562 [Pandoraea sp. XY-2]
MQIEFDPAKDEANREKHGLSLALAELFDLEAALLAIDERHDYDEERFVALGPLGDRLHVMVFTVRDESIRVISLRKANRR
EVKKYDEA

Nucleotide

• Length: 267 bp

>T108091 NZ_CP030849:c1697828-1697562 [Pandoraea sp. XY-2]
ATGCAAATCGAATTCGACCCAGCGAAAGACGAAGCGAACCGTGAGAAACATGGGCTGTCACTGGCGCTTGCAGAACTGTT
TGACCTTGAGGCTGCACTGCTGGCGATTGACGAGCGGCACGACTACGACGAGGAGCGATTCGTTGCGCTCGGCCCGCTTG
GCGACAGGCTCCATGTCATGGTGTTTACGGTGAGAGACGAGTCGATTCGAGTTATCAGTCTCCGCAAAGCTAATCGACGC
GAGGTGAAAAAGTATGACGAAGCGTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11863.35 Da
• Isoelectric Point: 7.2068

>AT108091 WP_130025489.1 NZ_CP030849:c1697552-1697232 [Pandoraea sp. XY-2]
MTDAEDAAITRAAETDSDSAPLSDEQLRKMRPAREVLSNVLGKERADAVLKRRGRPAMPESQRKVALNMRADRDVVEAFK
ATGDGWQTRMNEALREYAQSHHMLPA

Nucleotide

• Length: 321 bp

>AT108091 NZ_CP030849:c1697552-1697232 [Pandoraea sp. XY-2]
ATGACCGACGCTGAGGATGCTGCTATTACGCGTGCCGCTGAAACAGATTCGGATAGCGCACCGTTGAGCGATGAGCAGTT
GCGCAAGATGCGCCCCGCGCGCGAGGTGCTTTCCAATGTATTGGGTAAAGAGCGTGCCGATGCCGTGTTGAAGCGCCGGG
GACGCCCGGCAATGCCGGAGAGTCAACGAAAAGTGGCGCTCAACATGCGTGCCGACCGGGACGTGGTGGAAGCATTCAAA
GCCACCGGCGATGGTTGGCAAACGCGCATGAATGAGGCCCTGCGCGAGTATGCCCAGTCGCATCACATGTTGCCTGCTTG
A