Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 170556..171199 Replicon plasmid pAPEC-O2-211A-ColV
Accession NZ_CP030791
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag APECO2_RS27640 Protein ID WP_001034046.1
Coordinates 170783..171199 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag APECO2_RS27635 Protein ID WP_001261278.1
Coordinates 170556..170786 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS27600 166090..166395 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
APECO2_RS27605 166397..166615 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
APECO2_RS27615 167159..167671 + 513 WP_000151784.1 hypothetical protein -
APECO2_RS27620 167705..168838 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
APECO2_RS27625 169005..169778 - 774 WP_000905949.1 hypothetical protein -
APECO2_RS27630 169791..170291 - 501 WP_000528932.1 hypothetical protein -
APECO2_RS27635 170556..170786 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
APECO2_RS27640 170783..171199 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
APECO2_RS27645 171244..175038 - 3795 Protein_177 pcar -
APECO2_RS27650 175419..175649 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
APECO2_RS27655 175646..176062 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / vat / iutA / iucD / iucC / iucB / iucA 1..197773 197773


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T108072 WP_001034046.1 NZ_CP030791:170783-171199 [Escherichia coli APEC O2-211]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T108072 NZ_CP030791:170783-171199 [Escherichia coli APEC O2-211]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT108072 WP_001261278.1 NZ_CP030791:170556-170786 [Escherichia coli APEC O2-211]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT108072 NZ_CP030791:170556-170786 [Escherichia coli APEC O2-211]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References