Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 166090..166615 Replicon plasmid pAPEC-O2-211A-ColV
Accession NZ_CP030791
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag APECO2_RS27600 Protein ID WP_001159871.1
Coordinates 166090..166395 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag APECO2_RS27605 Protein ID WP_000813630.1
Coordinates 166397..166615 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS27585 162046..163251 - 1206 WP_001442122.1 AAA family ATPase -
APECO2_RS27590 163872..164603 + 732 WP_000504262.1 replication initiation protein -
APECO2_RS27595 165283..166089 - 807 WP_000016968.1 site-specific integrase -
APECO2_RS27600 166090..166395 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
APECO2_RS27605 166397..166615 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
APECO2_RS27615 167159..167671 + 513 WP_000151784.1 hypothetical protein -
APECO2_RS27620 167705..168838 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
APECO2_RS27625 169005..169778 - 774 WP_000905949.1 hypothetical protein -
APECO2_RS27630 169791..170291 - 501 WP_000528932.1 hypothetical protein -
APECO2_RS27635 170556..170786 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
APECO2_RS27640 170783..171199 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / vat / iutA / iucD / iucC / iucB / iucA 1..197773 197773


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T108071 WP_001159871.1 NZ_CP030791:c166395-166090 [Escherichia coli APEC O2-211]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T108071 NZ_CP030791:c166395-166090 [Escherichia coli APEC O2-211]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT108071 WP_000813630.1 NZ_CP030791:c166615-166397 [Escherichia coli APEC O2-211]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT108071 NZ_CP030791:c166615-166397 [Escherichia coli APEC O2-211]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References