Detailed information of TA system TA108062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 44623..44999
• Replicon: plasmid pAPEC-O2-211A-ColV
• Accession: NZ_CP030791
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: APECO2_RS26830
• Protein ID: WP_000969988.1
• Coordinates: 44623..44805 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: APECO2_RS26835
• Protein ID: WP_001324224.1
• Coordinates: 44802..44999 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS26815	42622..43656	-	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-
APECO2_RS26825	44216..44524	-	309	WP_000377483.1	hypothetical protein	-
APECO2_RS26830	44623..44805	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APECO2_RS26835	44802..44999	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
APECO2_RS26840	45681..46955	+	1275	WP_000489608.1	HlyD family secretion protein	-
APECO2_RS26845	46930..49044	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
APECO2_RS26850	49214..49525	-	312	WP_001259758.1	colicin V	-
APECO2_RS28140	49503..49739	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / vat / iutA / iucD / iucC / iucB / iucA	1..197773	197773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T108062 WP_000969988.1 NZ_CP030791:c44805-44623 [Escherichia coli APEC O2-211]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T108062 NZ_CP030791:c44805-44623 [Escherichia coli APEC O2-211]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT108062 WP_001324224.1 NZ_CP030791:c44999-44802 [Escherichia coli APEC O2-211]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT108062 NZ_CP030791:c44999-44802 [Escherichia coli APEC O2-211]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3