Detailed information of TA system TA108040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17683..18326
• Replicon: plasmid unnamed3
• Accession: NZ_CP030786
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AYN53_RS25345
• Protein ID: WP_053884725.1
• Coordinates: 17910..18326 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: AYN53_RS25340
• Protein ID: WP_059278859.1
• Coordinates: 17683..17913 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS25315	13021..13734	-	714	WP_072244077.1	putative DNA-binding domain-containing protein	-
AYN53_RS25320	13709..14560	-	852	WP_059278856.1	DUF692 domain-containing protein	-
AYN53_RS25325	14548..14865	-	318	WP_059236668.1	hypothetical protein	-
AYN53_RS25330	14869..15819	-	951	WP_113650876.1	MBL fold metallo-hydrolase	-
AYN53_RS25335	16648..17187	-	540	WP_125295819.1	hypothetical protein	-
AYN53_RS25340	17683..17913	+	231	WP_059278859.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AYN53_RS25345	17910..18326	+	417	WP_053884725.1	type II toxin-antitoxin system VapC family toxin	Toxin
AYN53_RS25350	18482..19291	+	810	WP_113650879.1	class I SAM-dependent methyltransferase	-
AYN53_RS25360	19967..20184	+	218	Protein_15	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AYN53_RS25365	20181..20517	+	337	Protein_16	VapC toxin family PIN domain ribonuclease	-
AYN53_RS25370	20567..22519	-	1953	WP_113650881.1	sigma 54-interacting transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papD / papC / vat / papD / papC	1..105846	105846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15079.42 Da
• Isoelectric Point: 7.8874

>T108040 WP_053884725.1 NZ_CP030786:17910-18326 [Escherichia albertii]
VNKTYMLDTNICSFIMREQPEAVIRRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGQLVDAFCARLDAILPWDRA
AVDATVEVKAALTAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPNLVLEDWVK

Nucleotide

• Length: 417 bp

>T108040 NZ_CP030786:17910-18326 [Escherichia albertii]
GTGAACAAAACGTATATGCTGGATACTAATATCTGCTCATTCATCATGCGTGAGCAGCCGGAGGCCGTGATCAGACGCTT
AGAGCAAGCGGTGCTGCGCAACCATCGCATCGTGGTCTCCGCTATTACCTACGCCGAAATGCGCTTCGGTGCTATCGGGA
AAAAAGCCTCGCCCCGTCATGGTCAACTGGTCGATGCGTTCTGCGCCCGCCTCGATGCGATTCTGCCGTGGGACCGTGCC
GCAGTCGATGCCACTGTAGAGGTCAAAGCAGCACTAACCGCAGCCGGTACACCAATCGGTCCCAACGACACAGCGATTGC
CGGACATGCTATAGCTGCAGGGGCCATTCTGGTGACCAATAATACGCGAGAGTTTGAGCGGGTGCCAAATTTGGTATTAG
AAGACTGGGTTAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8738.86 Da
• Isoelectric Point: 4.4692

>AT108040 WP_059278859.1 NZ_CP030786:17683-17913 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFLMEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT108040 NZ_CP030786:17683-17913 [Escherichia albertii]
ATGCGAACAGTATCTATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCTCGCGATCTGGATTTTGAGGGGGTGAG
TGAGTTGGAGATTGTCCGCGAAGGGGACAGCATCATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAGGCCGACGCAGACTTTTTGATGGAGCGTGAAGACGTGGTCAGTGACGAAGGACGATTTAACCTGTGA