Detailed information of TA system TA108037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 35574..36175
• Replicon: plasmid unnamed1
• Accession: NZ_CP030784
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: AYN53_RS24345
• Protein ID: WP_113650805.1
• Coordinates: 35795..36175 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AYN53_RS24340
• Protein ID: WP_001190712.1
• Coordinates: 35574..35795 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS24330	32654..33838	-	1185	WP_113650803.1	restriction endonuclease subunit S	-
AYN53_RS24335	33835..35391	-	1557	WP_113650804.1	type I restriction-modification system subunit M	-
AYN53_RS24340	35574..35795	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AYN53_RS24345	35795..36175	+	381	WP_113650805.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AYN53_RS24350	36180..36359	+	180	WP_000113018.1	hypothetical protein	-
AYN53_RS24355	36387..37430	+	1044	WP_113650806.1	DUF968 domain-containing protein	-
AYN53_RS24360	37519..37971	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
AYN53_RS24365	38058..39251	+	1194	WP_113650807.1	terminase	-
AYN53_RS24370	39251..40735	+	1485	WP_113650808.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..100347	100347
	-	flank	IS/Tn	-	-	40852..42039	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13664.39 Da
• Isoelectric Point: 5.1514

>T108037 WP_113650805.1 NZ_CP030784:35795-36175 [Escherichia albertii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVYSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T108037 NZ_CP030784:35795-36175 [Escherichia albertii]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCTGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATATTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT108037 WP_001190712.1 NZ_CP030784:35574-35795 [Escherichia albertii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT108037 NZ_CP030784:35574-35795 [Escherichia albertii]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACTCTGGACTCCACTAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6