Detailed information of TA system TA108033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3822351..3823151
• Replicon: chromosome
• Accession: NZ_CP030783
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AYN53_RS19465
• Protein ID: WP_095574314.1
• Coordinates: 3822351..3822878 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B1EHP0
• Locus tag: AYN53_RS19470
• Protein ID: WP_001277106.1
• Coordinates: 3822885..3823151 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS19440	3817426..3818194	-	769	Protein_3712	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AYN53_RS19445	3818191..3819468	-	1278	WP_113650493.1	branched chain amino acid ABC transporter permease LivM	-
AYN53_RS19450	3819465..3820391	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AYN53_RS19455	3820439..3821548	-	1110	WP_095574312.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AYN53_RS19460	3821971..3822354	+	384	WP_095574313.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AYN53_RS19465	3822351..3822878	-	528	WP_095574314.1	GNAT family N-acetyltransferase	Toxin
AYN53_RS19470	3822885..3823151	-	267	WP_001277106.1	DUF1778 domain-containing protein	Antitoxin
AYN53_RS19475	3823301..3824404	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AYN53_RS19480	3824676..3825530	-	855	WP_113650494.1	RNA polymerase sigma factor RpoH	-
AYN53_RS19485	3825775..3826833	-	1059	WP_113650495.1	permease-like cell division protein FtsX	-
AYN53_RS19490	3826826..3827494	-	669	WP_000617720.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19464.36 Da
• Isoelectric Point: 6.6263

>T108033 WP_095574314.1 NZ_CP030783:c3822878-3822351 [Escherichia albertii]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRACILCSNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVCSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T108033 NZ_CP030783:c3822878-3822351 [Escherichia albertii]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTTTGCGAGCGTGTATCCTTTGTAGCAACACTC
CAGAACGTCAGGTCCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGCTCAGCCTCATTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCTCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCGTTGGTTGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9942.37 Da
• Isoelectric Point: 10.0863

>AT108033 WP_001277106.1 NZ_CP030783:c3823151-3822885 [Escherichia albertii]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT108033 NZ_CP030783:c3823151-3822885 [Escherichia albertii]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAATCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGTTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z8E004