Detailed information of TA system TA108023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1943985..1944456
• Replicon: chromosome
• Accession: NZ_CP030783
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: AYN53_RS09955
• Protein ID: WP_001303511.1
• Coordinates: 1944178..1944456 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: AYN53_RS09950
• Protein ID: WP_001302048.1
• Coordinates: 1943985..1944176 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS09895	1939099..1939254	-	156	WP_113649980.1	type I toxin-antitoxin system toxin HokD	-
AYN53_RS09910	1939796..1940311	-	516	WP_059278955.1	hypothetical protein	-
AYN53_RS25895	1940308..1940484	-	177	WP_000753060.1	hypothetical protein	-
AYN53_RS09915	1940477..1940659	-	183	WP_059278956.1	hypothetical protein	-
AYN53_RS09920	1940753..1941109	-	357	WP_059278958.1	hypothetical protein	-
AYN53_RS09925	1941167..1941588	-	422	Protein_1896	DUF977 family protein	-
AYN53_RS09930	1941629..1942699	-	1071	WP_059278957.1	hypothetical protein	-
AYN53_RS09935	1942771..1943196	-	426	WP_001700667.1	hypothetical protein	-
AYN53_RS09940	1943193..1943495	-	303	WP_000172735.1	transcriptional regulator	-
AYN53_RS09945	1943593..1943964	+	372	WP_122985828.1	hypothetical protein	-
AYN53_RS09950	1943985..1944176	+	192	WP_001302048.1	hypothetical protein	Antitoxin
AYN53_RS09955	1944178..1944456	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYN53_RS09960	1944749..1944904	+	156	WP_072248456.1	DUF1391 family protein	-
AYN53_RS09965	1945064..1945282	+	219	WP_001171923.1	hypothetical protein	-
AYN53_RS09975	1945848..1946036	+	189	WP_000449200.1	hypothetical protein	-
AYN53_RS09980	1946033..1946224	+	192	WP_113649981.1	DUF1482 family protein	-
AYN53_RS09985	1946317..1948788	+	2472	WP_125295762.1	exonuclease	-
AYN53_RS09990	1948850..1949119	+	270	WP_059274360.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM2	1891872..1971745	79873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T108023 WP_001303511.1 NZ_CP030783:1944178-1944456 [Escherichia albertii]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T108023 NZ_CP030783:1944178-1944456 [Escherichia albertii]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT108023 WP_001302048.1 NZ_CP030783:1943985-1944176 [Escherichia albertii]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT108023 NZ_CP030783:1943985-1944176 [Escherichia albertii]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGCGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0