Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6680..7205 Replicon plasmid unnamed1
Accession NZ_CP030779
Organism Escherichia albertii strain 05-3106

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CYM74_RS23240 Protein ID WP_001159871.1
Coordinates 6680..6985 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CYM74_RS23245 Protein ID WP_000813634.1
Coordinates 6987..7205 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYM74_RS23220 1945..4155 + 2211 Protein_1 hypothetical protein -
CYM74_RS23230 4564..5314 - 751 Protein_2 replication initiation protein RepE -
CYM74_RS23235 5872..6679 - 808 Protein_3 phage integrase family protein -
CYM74_RS23240 6680..6985 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CYM74_RS23245 6987..7205 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CYM74_RS23255 7800..8069 + 270 WP_000343793.1 hypothetical protein -
CYM74_RS23260 8057..8653 + 597 WP_001277208.1 hypothetical protein -
CYM74_RS23270 9431..10408 + 978 WP_001414517.1 RepB family plasmid replication initiator protein -
CYM74_RS23275 10687..11427 - 741 WP_001066945.1 tyrosine-type recombinase/integrase -
CYM74_RS23280 11548..11682 - 135 WP_113623324.1 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bfpF / bfpE / bfpD / bfpA 1..56603 56603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T107967 WP_001159871.1 NZ_CP030779:c6985-6680 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107967 NZ_CP030779:c6985-6680 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT107967 WP_000813634.1 NZ_CP030779:c7205-6987 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107967 NZ_CP030779:c7205-6987 [Escherichia albertii]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References