Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2228034..2228610 Replicon chromosome
Accession NZ_CP030778
Organism Escherichia albertii strain 05-3106

Toxin (Protein)


Gene name shpA Uniprot ID A0A0K4FW98
Locus tag CYM74_RS11090 Protein ID WP_044708725.1
Coordinates 2228323..2228610 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0K4FU62
Locus tag CYM74_RS11085 Protein ID WP_054409941.1
Coordinates 2228034..2228336 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYM74_RS11060 2223572..2223703 - 132 WP_024164650.1 hypothetical protein -
CYM74_RS11065 2223721..2224060 - 340 Protein_2135 Tar ligand binding domain-containing protein -
CYM74_RS11070 2224560..2226710 + 2151 WP_044714865.1 pyruvate/proton symporter BtsT -
CYM74_RS11075 2226830..2227033 + 204 WP_000467859.1 YbdD/YjiX family protein -
CYM74_RS11080 2227044..2228000 + 957 WP_059235079.1 GTPase -
CYM74_RS11085 2228034..2228336 - 303 WP_054409941.1 BrnA antitoxin family protein Antitoxin
CYM74_RS11090 2228323..2228610 - 288 WP_044708725.1 BrnT family toxin Toxin
CYM74_RS11095 2228807..2229973 + 1167 WP_000800832.1 restriction endonuclease -
CYM74_RS11100 2230037..2231656 + 1620 WP_044717122.1 type I restriction-modification system subunit M -
CYM74_RS11105 2231646..2233046 + 1401 WP_059254411.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11155.59 Da        Isoelectric Point: 6.9903

>T107954 WP_044708725.1 NZ_CP030778:c2228610-2228323 [Escherichia albertii]
MPMEFEWDANKAKSNQVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T107954 NZ_CP030778:c2228610-2228323 [Escherichia albertii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCAGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTACACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.04 Da        Isoelectric Point: 9.4646

>AT107954 WP_054409941.1 NZ_CP030778:c2228336-2228034 [Escherichia albertii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDELWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT107954 NZ_CP030778:c2228336-2228034 [Escherichia albertii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCTATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4FW98

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4FU62

References