Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 176829..177388 | Replicon | plasmid pINV-Y394 |
| Accession | NZ_CP030773 | ||
| Organism | Shigella flexneri 1c strain Y394 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | B7485_RS24565 | Protein ID | WP_000421262.1 |
| Coordinates | 177113..177388 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | B7485_RS24560 | Protein ID | WP_024260696.1 |
| Coordinates | 176829..177113 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B7485_RS24520 (B7485_26555) | 172309..172659 | + | 351 | WP_005048975.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| B7485_RS24525 (B7485_26560) | 172656..173207 | + | 552 | Protein_206 | IS66 family transposase zinc-finger binding domain-containing protein | - |
| B7485_RS24530 (B7485_26565) | 173265..174421 | + | 1157 | WP_134796986.1 | IS3 family transposase | - |
| B7485_RS24535 (B7485_26570) | 174465..175394 | + | 930 | Protein_208 | IS66-like element ISSfl3 family transposase | - |
| B7485_RS24540 (B7485_26575) | 175583..175768 | + | 186 | Protein_209 | ATP-binding protein | - |
| B7485_RS25890 (B7485_26580) | 175868..176073 | - | 206 | Protein_210 | peptidoglycan-binding protein | - |
| B7485_RS24550 (B7485_26585) | 176126..176377 | - | 252 | WP_001381727.1 | transporter | - |
| B7485_RS24560 (B7485_26595) | 176829..177113 | + | 285 | WP_024260696.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| B7485_RS24565 (B7485_26600) | 177113..177388 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| B7485_RS24580 (B7485_26615) | 178367..179320 | + | 954 | Protein_215 | IS66 family transposase | - |
| B7485_RS24585 (B7485_26620) | 179856..180074 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| B7485_RS24590 (B7485_26625) | 180076..180381 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| B7485_RS24595 (B7485_26630) | 180405..180512 | + | 108 | WP_023592908.1 | transposase domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE | 1..221293 | 221293 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 165231..187205 | 21974 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T107932 WP_000421262.1 NZ_CP030773:177113-177388 [Shigella flexneri 1c]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T107932 NZ_CP030773:177113-177388 [Shigella flexneri 1c]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10513.93 Da Isoelectric Point: 6.2150
>AT107932 WP_024260696.1 NZ_CP030773:176829-177113 [Shigella flexneri 1c]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT107932 NZ_CP030773:176829-177113 [Shigella flexneri 1c]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|