Detailed information of TA system TA107931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 86893..87650
• Replicon: plasmid pINV-Y394
• Accession: NZ_CP030773
• Organism: Shigella flexneri 1c strain Y394

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: B7485_RS23975
• Protein ID: WP_128882125.1
• Coordinates: 86893..87378 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: B7485_RS23980
• Protein ID: WP_011114751.1
• Coordinates: 87366..87650 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7485_RS23955 (B7485_25990)	81963..83654	-	1692	WP_149528155.1	IpaH family type III secretion system E3 ubiquitin--protein ligase	-
B7485_RS23965 (B7485_26000)	85216..86372	-	1157	Protein_104	IS3-like element IS600 family transposase	-
B7485_RS23970 (B7485_26005)	86439..86720	+	282	Protein_105	IS4/IS5 family transposase	-
B7485_RS23975 (B7485_26010)	86893..87378	-	486	WP_128882125.1	GNAT family N-acetyltransferase	Toxin
B7485_RS23980 (B7485_26015)	87366..87650	-	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
B7485_RS25315	88552..88716	+	165	WP_001346193.1	hypothetical protein	-
B7485_RS24005 (B7485_26040)	88970..90572	-	1603	Protein_109	IS66-like element ISSfl3 family transposase	-
B7485_RS24010 (B7485_26045)	90569..90919	-	351	WP_005048975.1	IS66 family insertion sequence element accessory protein TnpB	-
B7485_RS24015 (B7485_26050)	90916..91590	-	675	WP_001088287.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE	1..221293	221293
	-	inside	IScluster/Tn	-	ospC2 / ipaH2.5 / ospC3	72277..99580	27303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17770.62 Da
• Isoelectric Point: 10.1083

>T107931 WP_128882125.1 NZ_CP030773:c87378-86893 [Shigella flexneri 1c]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PAPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T107931 NZ_CP030773:c87378-86893 [Shigella flexneri 1c]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGCCCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT107931 WP_011114751.1 NZ_CP030773:c87650-87366 [Shigella flexneri 1c]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT107931 NZ_CP030773:c87650-87366 [Shigella flexneri 1c]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4V1CUJ1